Compare the lysis for bacteria to that for CHO (Chinese Hamster Ovary) cells. Note, CHO cells are commonly used for production of therapeutics. How resistant are CHO cells compared to the bacterial cells? If the same level of disruption is expected for CHO cells and the homogenizer is operated at the same pressure for both types of cells, would you expect CHO cells to require more or less number of passes compared to E.coli or Bacillis brevis cells?
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
-
Compare the lysis for bacteria to that for CHO (Chinese Hamster Ovary) cells. Note, CHO cells are commonly used for production of therapeutics.
-
How resistant are CHO cells compared to the bacterial cells?
-
If the same level of disruption is expected for CHO cells and the homogenizer is operated at the same pressure for both types of cells, would you expect CHO cells to require more or less number of passes compared to E.coli or Bacillis brevis cells?
-
Step by step
Solved in 2 steps
