Please explain how different detergents take out proteins selectively out of membrane. (Choose one protein as an example from the figure)

Human Anatomy & Physiology (11th Edition)
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Please explain how different detergents take out proteins selectively out of membrane. (Choose one protein as an example from the figure)
top
bottom
top
bottom
top
bottom
VIP17/MAL
PLAP
caveolin-1
stomatin
Yes
TfR
gp114
calnexin
rab-5
Lubrol WX
Brij 96
Triton
association of marker proteins from MDCK cells. MDCK-PLAP cell homogenate was extracted with 0.5% Lubrol WX, 0.5% Brij 96
n linear sucrose gradients. The distribution of marker preins was analyzed by immunoblotting. The light fractions from the top c
t, the heavy bottom fractions on the right.
ally solubilized gp114, most of TfR, and 50% of Yes
ter), whereas Triton solubilized rab-5, calnexin, gp114,
nost of Yes (Fig. 2 Right). Hence, DRM association of
ker proteins confirms the graded selectivity of the
observed by analysis of the plasma membrane DRM
ntent.
ults with Triton agreed with earlier observations (6, 11,
Regarding Lubrol WX, calnexin had previously been
phospholipid and cholesterol/glycerophospholipid
calculated. The fold changes of these ratios relative t
are depicted in Fig. 4. No significant changes occ
higher detergent concentrations were used or wi
where prepared by pelleting from isolated membrane
flotation from cell homogenate (not shown).
Compared with TCMS, DRMS from MDCK cell
with Triton and CHAPS showed clear enrichment c
Transcribed Image Text:top bottom top bottom top bottom VIP17/MAL PLAP caveolin-1 stomatin Yes TfR gp114 calnexin rab-5 Lubrol WX Brij 96 Triton association of marker proteins from MDCK cells. MDCK-PLAP cell homogenate was extracted with 0.5% Lubrol WX, 0.5% Brij 96 n linear sucrose gradients. The distribution of marker preins was analyzed by immunoblotting. The light fractions from the top c t, the heavy bottom fractions on the right. ally solubilized gp114, most of TfR, and 50% of Yes ter), whereas Triton solubilized rab-5, calnexin, gp114, nost of Yes (Fig. 2 Right). Hence, DRM association of ker proteins confirms the graded selectivity of the observed by analysis of the plasma membrane DRM ntent. ults with Triton agreed with earlier observations (6, 11, Regarding Lubrol WX, calnexin had previously been phospholipid and cholesterol/glycerophospholipid calculated. The fold changes of these ratios relative t are depicted in Fig. 4. No significant changes occ higher detergent concentrations were used or wi where prepared by pelleting from isolated membrane flotation from cell homogenate (not shown). Compared with TCMS, DRMS from MDCK cell with Triton and CHAPS showed clear enrichment c
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