One ml of a sample was added to 99 ml of buffer. 100 µ of this was plated in nutrient agar. After incubation, 247 colonies were present on the plate. How many colony-forming units were present per ml of the original sample? Show your working out. What would you record in your workbook if there were 378 colonies present? Why? B. Give definitions of these bacterial growth in relation to O2 availability. 1. E. coli , micrococcus Letues, clostridium sprogenes

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Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.