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Two different bacterial samples, A and B, were inoculated onto SIM media. What can you conclude based on your observations? A

Two different bacterial samples, A and B, were inoculated onto SIM media. What can you conclude based on your observations? A

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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**Question:** Based on your observation of the pictured specimens that were inoculated into SIM tubes, select ALL appropriate conclusions. **Options:** - [ ] Specimen A produced indole - [ ] Specimen A did not produce indole - [ ] Specimen A produced hydrogen sulfide (H₂S) - [ ] Specimen A is motile - [ ] Specimen B produced indole - [ ] Specimen B did not produce indole - [ ] Specimen B did not produce hydrogen sulfide (H₂S) - [ ] Specimen B is motile **Explanation:** Students should select all conclusions that are supported by their observation of the SIM tubes in the experiment. The SIM (Sulfide Indole Motility) test is used in microbiology to assess three differential characteristics of bacteria: sulfur reduction, indole production, and motility.
Transcribed Image Text:**Question:** Based on your observation of the pictured specimens that were inoculated into SIM tubes, select ALL appropriate conclusions. **Options:** - [ ] Specimen A produced indole - [ ] Specimen A did not produce indole - [ ] Specimen A produced hydrogen sulfide (H₂S) - [ ] Specimen A is motile - [ ] Specimen B produced indole - [ ] Specimen B did not produce indole - [ ] Specimen B did not produce hydrogen sulfide (H₂S) - [ ] Specimen B is motile **Explanation:** Students should select all conclusions that are supported by their observation of the SIM tubes in the experiment. The SIM (Sulfide Indole Motility) test is used in microbiology to assess three differential characteristics of bacteria: sulfur reduction, indole production, and motility.
### Observing Bacterial Samples Inoculated onto SIM Media #### Experimental Setup Two different bacterial samples, labeled A and B, were inoculated into SIM (Sulfide Indole Motility) media to observe potential biochemical activities. #### Observations In the image, two test tubes labeled A and B are shown: - **Sample A (Left Tube)**: - The medium has a distinct red color at the top. - It also shows the yellow coloration throughout the medium. - A small red spot is apparent in the middle of the yellow medium. - **Sample B (Right Tube)**: - The medium appears uniformly yellow with no obvious red or black coloration. #### Interpretation of Results **Sample A**: 1. **Indole Production**: The presence of a red color at the top of the medium indicates a positive indole test, suggesting that this bacterial sample can break down tryptophan to produce indole. 2. **Hydrogen Sulfide Production**: There is no black precipitate observed, which suggests a negative result for hydrogen sulfide (H₂S) production. 3. **Motility**: The red diffusion in the middle of the yellow medium indicates that the bacteria may be motile, as they seem to have moved away from the stab line. **Sample B**: 1. **Indole Production**: Since there is no red color at the top of the medium, the indole test is negative, indicating that this bacterial sample does not produce indole. 2. **Hydrogen Sulfide Production**: The absence of a black precipitate suggests a negative result for hydrogen sulfide (H₂S) production. 3. **Motility**: The uniform yellow appearance indicates that the bacteria are non-motile, as there is no growth away from the stab line. #### Conclusion Based on the observations: - **Sample A** is indole positive, non-H₂S producing, and motile. - **Sample B** is indole negative, non-H₂S producing, and non-motile. #### Next Steps 1. Further confirmatory tests to validate these observations. 2. Consideration of additional biochemical tests for comprehensive identification of bacterial species. #### Related Resources - [SIM Medium Explanation and Interpretation](URL to additional resource) - [Understanding Bacterial Morphology and Motility](URL to additional resource) **View the image in greater detail.** #### Exercise
Transcribed Image Text:### Observing Bacterial Samples Inoculated onto SIM Media #### Experimental Setup Two different bacterial samples, labeled A and B, were inoculated into SIM (Sulfide Indole Motility) media to observe potential biochemical activities. #### Observations In the image, two test tubes labeled A and B are shown: - **Sample A (Left Tube)**: - The medium has a distinct red color at the top. - It also shows the yellow coloration throughout the medium. - A small red spot is apparent in the middle of the yellow medium. - **Sample B (Right Tube)**: - The medium appears uniformly yellow with no obvious red or black coloration. #### Interpretation of Results **Sample A**: 1. **Indole Production**: The presence of a red color at the top of the medium indicates a positive indole test, suggesting that this bacterial sample can break down tryptophan to produce indole. 2. **Hydrogen Sulfide Production**: There is no black precipitate observed, which suggests a negative result for hydrogen sulfide (H₂S) production. 3. **Motility**: The red diffusion in the middle of the yellow medium indicates that the bacteria may be motile, as they seem to have moved away from the stab line. **Sample B**: 1. **Indole Production**: Since there is no red color at the top of the medium, the indole test is negative, indicating that this bacterial sample does not produce indole. 2. **Hydrogen Sulfide Production**: The absence of a black precipitate suggests a negative result for hydrogen sulfide (H₂S) production. 3. **Motility**: The uniform yellow appearance indicates that the bacteria are non-motile, as there is no growth away from the stab line. #### Conclusion Based on the observations: - **Sample A** is indole positive, non-H₂S producing, and motile. - **Sample B** is indole negative, non-H₂S producing, and non-motile. #### Next Steps 1. Further confirmatory tests to validate these observations. 2. Consideration of additional biochemical tests for comprehensive identification of bacterial species. #### Related Resources - [SIM Medium Explanation and Interpretation](URL to additional resource) - [Understanding Bacterial Morphology and Motility](URL to additional resource) **View the image in greater detail.** #### Exercise
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