Researchers discover a new strain of a previously known bacterial organism and need helpunderstanding properties related to it's bacterial growth patterns. The new strain is lethal to humansand is found to be increasingly deadly in arctic climates. Researchers have also provided thefollowing chart of plate counts:Colony|(minutes) FormingUnits by ml(CFU/ml)Time201520303404550,00960175,168,18590175,168,189120175,168,002150423,237210505| 2705a) Create a bacterial growth curve with log values of CFU/ml on the Y-axis andthe timeon the x axis.b) Indicate the different phases of the growth curve on your plot.c) Determine the growth constant value (k) and the Generation Time value (G).show calculations8 8

Bacterial growth curve is a graphical representation of number of live bacterial cells over the time…

Answered: a) Create a bacterial growth curve with…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

1a.) How pH of the culture medium affects the cell culture? Please give examples of low pH and high-pH culture conditions. 1b.) Describe the difference between defined and undefined cell culture media. What are the advantages and disadvantages of both of them?
Indicate which are the laboratory conditions necessary to cultivate Clostridium bacteria in medium, atmosphere and incubation time
What method of bacterial measurement does this graph represent? Is it considered direct, or indirect? What phase of the growth curve can NOT be measured using this method?
You have several different media onto which you inoculated eight strains of yeast (A-H). The media include a rich medium, an unsupplemented minimal medium, and minimal media each supplemented with one vitamin. Of the yeast strains, one is a prototroph and seven are auxotrophs for a vitamin. After overnight incubation, the following results were observed (tan patches represent growth): D plate 1 (A) B DE F GH plate 5 plate 4 plate 6 Which plate contains an unsupplemented minimal medium? [Select] Which plate contains a rich medium? [Select] plate 2 Which strain is a prototroph? [Select] Strain E is an auxotroph for niacin. Which plate reveals this specific auxotrophy? [ Select] plate 3 plate 7 One strain is an auxotroph for both choline and pantothenic acid. Which one is this most likely to be? [Select]
procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (1) broth culture to broth
MSA (Mannitol Salt Agar) Match the description to the correct Bacterium A= growth, agar is yellow B= Growth, agar is not yellow C= no growth
A culture consisting of 100 litres of nutrient medium containing 12 g l-1 of growth limiting substrate is inoculated with 10g of bacteria in a batch fermenter. The yield of biomass from substrate is 0.05 g g-1 . The maintenance coefficient is 2.0 g g-1 h -1 . The specific rate of product formation due to maintenance is 1.0 h-1 . The maximum specific growth rate of the organism is 0.3 h -1 . YPX= 7.7 and product formation is directly linked with metabolism. a) What is the time required to produce 15 g of biomass? !!!!! ANS IS 3.07 HRS!!!! STEPS on how to reach this please
A physician obtains a specimen from a patient with tuberculosis. This sample has one viable bacteria, an organism with slow doubling time in vitro of 48 hours, which corresponds to the growth rate constant in vitro of 0.04 h^-1. Estimating that the biomass of a single mycobacterial organism is 2.3x10^-13 g and that this organism is immediately going to enter the log phase, how many hours will it take to produce 10^-6 grams of biomass?
4. (a) Which of the following chemical reactions is an overall oxidation-reduction (redox) reaction that involves oxidation number changes in their reactions? A. NaOH + H₂O → Na¹ + H3O+ + OH B. HCl + H₂O → H3O+ + Cl- C. CO₂ + H₂O → H+ + CO3²- D. N₂ + 3H₂ → 2NH3
All of the following are valid measurements of growth except: A. Counting viable cells B. Estimating the weight of cells C. Determining the dry weight of cells D. Streaking for single colonies E. Measuring some cellular component F. Counting the total number of cells
Strain X. spp. is a rod-shaped, anaerobic, Gram-positive bacterium with peritrichous flagella and pili that could use glucose and dye as its sole carbon source. Answer the following sections (a, b and c). a) With a doubling time of 240 minutes and a starting population size of 10 bacteria cells. How many bacteria cells will be present after 72 hours, assuming no cell death? b) Draw the diagram and describe motility required for the bacteria to gradient concentration of attractant (food)? c) Draw the diagram of the up flow packed-bed column biofilm reactor under continuous operation and label completely
a. What oxygen requirements do your think this bacterium will have (obligate aerobe, obligate anaerobe, facultative anaerobe, aerotolerant anaerobe, or microaerophile), and why? b. You run a thioglycollate test of your isolated bacterium. Based on your response to Part A, which thioglycollate tube best represents the result you would expect for your bacterium? Explain your response. If the result you would expect is not represented, explain what the tube should look like. c. What would you expect to be the result of a catalase test of your bacterium? d. What techniques or specialized equipment, if any, would you need to grow cultures of this bacterium?

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