A Lactobacillus casei population of 4x105 multiplies without lag phase to 3.68x107 in 6 h without having reached the stationary phase. What is the generation time of the microorganism?
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A Lactobacillus casei population of 4x105 multiplies without
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- If the generation time of Bacillus cereus is 28 minutes, how many cells of B. cereus will there be after an 90 minutes?Strain X. spp. is a rod-shaped, anaerobic, Gram-positive bacterium with peritrichous flagella andpili that could use glucose and dye as its sole carbon source. Answer the following sections (a, band c).a) With a doubling time of 240 minutes and a starting population size of 10 bacteria cells. Howmany bacteria cells will be present after 72 hours, assuming no cell death?b) Draw the diagram and describe motility required for the bacteria to gradient concentrationof attractant (food)?Strain X. spp. is a rod-shaped, anaerobic, Gram-positive bacterium with peritrichous flagella and pili that could use glucose and dye as its sole carbon source. Answer the following sections (a, b and c). a) With a doubling time of 240 minutes and a starting population size of 10 bacteria cells. How many bacteria cells will be present after 72 hours, assuming no cell death? b) Draw the diagram and describe motility required for the bacteria to gradient concentration of attractant (food)? c) Draw the diagram of the up flow packed-bed column biofilm reactor under continuous operation and label completely
- A culture of S. cerevisea has an overnight OD of 4.5 (1.0 OD is approx 1.0x107 cells/ml) You will be plating 100µl onto agar and want the final count of colonies on the plate to be around 300 colonies. How much of the 4.5 OD culture must you use to get a 500µl subdilution (with sterile water), so that you have diluted enough to get approx 300 colonies per 100ulYou are growing a culture of S. aureus. You know that 9 generations have occurred after 6 hours. What is the generation time in minutes? if i started a culture with 1000 cells, after 8 generations, how many cells will i have?describe the liquid growth analysis in detail.
- If the number of bacteria were to grow exponentially with a daily rate of r = 0.2612, how long would it take for the number of cases to double? Explain.N. benthamiana will be infiltrated with a solution containing OD600=0.3 of each experimental Agro containing construct and OD600=0.1 of p19. Calculate the volume of cultures (V construct; V p19) needed according to the formulas: V construct = V final × 0.3/OD600; V p19 = V final × 0.1/OD600. One mL of infiltrate is often enough to complete a small experiment. How to plan your final volume accordingly?You have a starter culture containing 8 x 109 cells per mL, from which you take 10mL to inoculate a fresh 1 L culture. After 15 hours, the cell density of the new culture is 3 x 1012cells per mL. What are the generation time and the mean growth rate constant of theorganism in culture?
- If a flask is inoculate with 10 E. coli cells. After 6 hours you now have 200,000 cells. determine both n (number of generations) and g (generation time) in minutes? A culture of S. epidermidis is growing. It divides every 20 min. After 4 hours, how many generations occurred?Use the following passage to answer 2 consecutive questions. A urine specimen was collected in a container and placed on the central desk for delivery at 10:00 am. The specimen is not sent to the lab until 5 hours later (3:00 pm). The sample time was marked by the central clerk as collected at 4:00 pm. The bacterial species has a generation time of 30 minutes at room temperature. There are 100 bacterial cells/ml at the time of collection (10:00 am). The laboratory will cite a bacterial infection at more than 1000 bacterial cells/ml.A physician obtains a specimen from a patient with tuberculosis. This sample has one viable bacteria, an organism with slow doubling time in vitro of 48 hours, which corresponds to the growth rate constant in vitro of 0.04 h^-1. Estimating that the biomass of a single mycobacterial organism is 2.3x10^-13 g and that this organism is immediately going to enter the log phase, how many hours will it take to produce 10^-6 grams of biomass?