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- explain how the use of an Eosin methylene blue agar plate can help determine the type of fermenters that bacteria are. explain throughly.Explain the activities associated with the isolation of Bacillus subtilis organsim from mixed culture to pure culture. Include the relevant information( type of inoculation, source of bacteria etc)? Also the isolation of the organism from the broth containing a fresh mixture of 2 or more bacteria was obtained after incubation of nutreint agar plate.Describe colonial characteristics of the organisms grown on the different culture media according to its reaction to pigmentation, lactose fermentation and hemolysis. Nutrient Agar Blood Agar MacConkey EMB Identify only if pigmented or non- pigmented • Identify hemolytic reactions present • Other characteristic if any Identify if lactose Identify if lactose fermenter or non- fermenter or non- Microorganisms lactose lactose fermenters fermenters Other characteristics if any 1. Escherichia coli 2. Klebsiella pneumoniae 3. Proteus spp. 4. Pseudomonas aeruginosa Page 3 I 3 Q + B.
- Explain the activities associated with the isolation of Bacillus megaterium organsim from mixed culture to pure culture. Include the inoculum source, incubation temperature and other information.Complete the table below with the observations: include amount of growth (-, +, ++, +, ++), colonial morphology for NA, color of colonies for EMB, and media color for MSA. Organism/ Media Nutrient Agar (NA) Eosin Methylene Blue (EMB) Agar Mannitol Salt Agar (MSA) Escherichia coli Pseudomonas aeruginosa Staphylococcus epidermidis Staphylococcus aureus Unknown 9 mixtureAnswer the following questions briefly and concisely 1.How do bacteria in a chemostat and those in a batch culture vary from one another? 2. What happens in a chemostat if the dilution rate is higher than the organism's maximum specific growth rate? 3.Does a chemostat require the use of pure cultures? 4. Why would a complicated culture media for Leuconostoc mesenteroides be simpler to make than one with a fixed chemical composition?
- What is the major difference between an enrichment culture and a selective culture? Why are microbial doubling times in nature typically longer than those obtained in the lab? Briefly describe the following mechanisms of measuring bacterial growth: Direct microscopic cell count Plate count Most probable numberDifferentiate the various types of culture media for the growth ofmicroorganisms: synthetic, complex, defined, selective and differential.Explain the activities associated with the isolation of Bacillus subtilis organsim from mixed culture to pure culture. Include the inoculum source, incubation temperature and other information.
- Can you please discuss the principles of using Motility Test Medium for the detection of bacterial motility. Focus on the components of the medium and the purpose of each component. Note: You may refer to different culture media manuals like Difco, BBL, etc.Identify culture methods and conditions used for blood, wound,urine, fecal, and genital specimens. Of what importance areselective and differential growth media in pathogen detection,and what special conditions must be maintained for theisolation of anaerobic pathogens?ORGANISMS: STENOTROPHOMONAS MALTOPHILIA Outline the presumptive and definitive characteristics for the laboratory identification of the bacterial species being presented. Discuss the diagnostic and clinical implications of making correct organism identification by giving follow up tests.