Lidocaine HCl (E = 0.2) 0.60g Boric Acid (E = 0.52) 0.03g Sterile water q.s. 30ml Make isoton. sol. Use as directed by physician.
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1. How many mL of sterile water must be added to make the preparation isotonic?
2. How many mL of another isotonic solution must be added to make the final volume of the solution?

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- HYPERTONIC SOLUTION Water Solute 98% Water Solute Cell Condition:9. Which is statement below is the correct definition of the Sterility Assurance Limit (SAL). Select ONE answer. O The Sterility Assurance Limit (SAL) is a numerical value that predicts the probability that a microorganism has survived a pasturization process. O The Sterility Assurance Limit (SAL) is a numerical value that confirms that a microorganism has survived a sterilisation process. O The Sterility Assurance Limit (SAL) is a numerical value that predicts the probability thaț a microorganism has survived a sterilisation process. O The Sterility Assurance Limit (SAL) is a numerical value that confirms that a microorganism has survived a pasturization process. O The Sterility Assurance Limit (SAL) is a numerical value that predicts the probability that a microorganism has been erradicated during a sterilisation process3. How would you use a 3-step serial dilution to produce 1ml of a luM Glucose solution from a 1M Glucose stock? What would be the total dilution factor?
- 1. Determine the inactivation rate constant (K) for a microorganism for the following treated effluent sample. The effluent temperature was 20°C. If the activation energy for the disinfection reaction is 60 kJ/mole, determine the inactivation rate constant (K) at 12°C. Assume Chick's law applies. In (N/N.) Time, min 1.5 3.9 3 2.5 5.6 7.7 9.214. You need to load 25 µg of protein into one of the wells of a gel. This needs to be in 1X buffer and in a total volume of 25 µL. You are given a 1.25 µg/µL solution of protein, a 5X buffer, and water. How much of each should you mix together in order to correctly prepare the sample you need to load?Which solution is most hypertonic? And which solution is most hypotonic?
- 20. According to the package insert, adding 2.5 mL of sterile water to a 1 g cefazolin vial yields a final volume of 3 mL. What volume of this solution would you need to obtain 200 mg of cefazolin?1. Which substances are able to diffuse through dialysis tubing? Choose all that apply. - Protein - Glucose - Sodium chloride - Starch 2. In yeast, which culture(s) are able to demonstrate diffusion? (Choose one option below) A) Only the dead yeast culture demonstrated diffusion B) Both the alive and dead yeast culture demonstrated diffusion C) Only the alive yeast culture demonstrated diffusion D) Neither the alive nor dead yeast culture demonstrated diffusion 3. In yeast, which culture(s) demonstrate active transport? (Choose one option below) A) Only the alive yeast culture demonstrated active transport B) Neither the alive nor dead yeast culture demonstrated active transport C) Only the dead yeast culture demonstrated active transport D) Both the alive and dead yeast culture demonstrated active transport 4. In yeast, adenosine triphosphate (ATP) does not provide the energy for active transport to occur (True or False?) A) True B) False1.What advantage(s) does the pour plate method have over the streak-plate method? 2.Why is the loop flamed before it is placed in a culture tube? Why is it flamed after completing the inoculation? 3.Before inoculating and pouring molten nutrient agar into a plate, why must the agar first be cooled to 50° C? 4.Explain why plates should be inverted during incubation. 5.Explain why plates should be inverted during incubation.
- GELATIN b.how will you inoculate? c.How will you incubate? d. what are Post- Incubation procedures? e. How will you read the positive and negative results?Which of the following correctly matches the chromatography technique with the molecular property being exploited for separation? ion-exchange: charge,affinity: polarity, gel-filtration: molecular mass ion-exchange: charge, affinity:molecular binding preference,gel-filtration: molecular size ion-exchange: charge,affinity: molecular binding preference,gel-filtration: isoilectric point ion-exchange: ion binding preference,affinity: molecular size,gel-filtration:polarity11. On the basis of the indicator test for starch what must have happened to the iodine molecules that were originally only in the water in the large test tube?