5. We’re back in the lab having fun! Our current experiment calls for us to treat our cells with THC (yeet!, delta 8 from hemp of course lol) and the final concentration of 15 µM once it has been added to the cells. We need to treat 10 mL of cells, and we don't want our treatment volume to be more than 10 µL per 1 mL of cells. What concentration should we make our stock solution?

Q: III. True or False   a. Large proteins travel/migrate faster in both gel filtration chromatography…

A: Different techniques used in the separation and purification of the biomolecules are, Chromatography…

Q: What are the different methods of cell lysis? Discuss in detail.

A: Common ways to perform cell lysis include using pressure, temperature changes, ultrasonic…

Q: Blood agar is a differential medium that will show us a clear zone around a colony of normal flora…

A: A differential medium is made up of chemicals or composition that would help us to determine…

Q: Explain possible explanations for the differences noted among the diffusion rates of the three…

A: Among the few factors that affect the diffusion rate, molecular weight is the major one. As is…

Q: 1. The concentration of the glucose stock solution in bottle is 50 µM. This stock solution was…

A: The concentration of the solution was measured by C1V1=C2V2 Whereas, C1 = Concentration of stock…

Q: 2 a. You are trying to purify protein C from a mixture of proteins noted in the above Table. If you…

A: Chromatography is biochemical separation method for organic molecules or solutes of a compound…

Q: 1. The chromatography solvent is very polar as it contains alcohol, an acid and water. Based on this…

A: Chromatography is a separation technique that is used to separate components of a mixture based on…

Q: 11. Review the following experiment and answer the questions. You set up an experiment where you…

A: Dialysis: it is a process to remove the waste material of the body in patients with kidney…

Q: The first choice : ori lacZ blaz araC GFP The second choice : arabinose Ampicillin  X-gal…

A: In molecular cloning experiments, one of the essential techniques employed is the "Blue and White…

Q: a. Why might this be particularly true when comparing highly volatile chemicals like alcohol to less…

A: Answer: ANTI-BACTERIAL = These are the chemicals  which have capability to inhibit or kill the…

Q: sphingolipids

A: Plasmogens:  The plasmogen  is the chemical substance which is the  important living portion of…

Q: Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) 1. Why do you destain the gel?

A: There are various electrophoresis techniques used for the separation and identification of proteins.…

Q: 1- Are the following changes in conditions likely to increase or decrease retention time? a. faster…

A: "Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: You have are diluting down a concentrated stock of bacteria in order to grow them. Starting with 2.1…

A: A thick solution can be made into a more useful concentration by performing a series of sequential…

Q: hic water cóntains properties that can treat a disease transmitted by nat type of vector?

A: Tonic water treat malaria which is caused by mosquitoes which acts as a vector.  So first option is…

Q: Which of the following polymer is intracellular? chondroitin sulfate Heparan sulfate Keratan…

A: A polymer is a big molecule made up of smaller building blocks called monomers that repeat and come…

Q: 3. (a) (b) What are the different methods of cell lysis? Discuss in detail. In Streptomyces…

A: 3.a)Cell lysis is the process of breaking down the cell in order to extract desired product which is…

Q: Which of these disinfectants is least effective against E. coli? a. Chlorine b. o-phenylphenol c.…

A: Disinfectants: These are the chemical compounds which are applied on the non living objects to kill…

Q: 3. (a) What are the different methods of cell lysis? Discuss in detail.

A: Cell disruption refers to the process of breaking cells in order to obtain the desired product,…

Q: 3. How many milliliters would you need to prepare a 10-2 dilution from a 10ml starting culture?

A: Sometimes actual culture contains too many generations of microorganisms because of which the colony…

Q: What are the different methods of cell lysis? Discuss in detail. In Streptor filtration from an orth…

A: Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: 6. A solution made up of 500 mL of 50% dextrose, 500 mL of 8.5% Aminosyn, and 300mL of sterile water…

A: Dextrose, often known as glucose is a monosaccharide carbohydrate and a simple sugar. It is a…

Q: (a) What are the different methods of cell lysis? Discuss in detail. (b) In Streptomyces filtration…

A: (a) Both animal and plant cells are covered by a lipoprotein membrane called cell membrane.…

Q: ou have a polypeptide chain that is linked by disulfide bond. If beta-mercaptoethanol is applied,…

A: A polypeptide chain that is linked by disulfide bond. If beta-mercaptoethanol is applied, there is…

Q: 3. Identify the IMVIC pattern of the following tubes. For set of tubes that is E. coli positive,…

A: The IMViC tests are a collection of separate assays used in microbiological labs to detect coliform…

Q: hoose a specific drug name (NOT A DRUG CLASS) under CELL WALL SYNTHESIS INHIBITORS, and DRAW the…

A: Answer for the following question are :

Q: You have an order for 125000 units of an antibiotic IM stat. The directions state to reconstitute…

A: Antibiotics are medications that are used to treat bacterial infections. Antibiotics either kill the…

Q: Draw two water molecules making hydrogen bonds with the covid19 drug remdesivir Remdisivir NH2 HN…

A:

Q: O 5 P.A S.A. 2.

A: In this method paper disc saturated with a specific anti microbial agent one at a time is placed on…

Q: 6. Imagine the experiment below where you placed a dialysis tube (cell) containing 10% NaCl Solution…

A: Osmosis is the process where solvent/liquid moves through semipermeable membrane unless both the…

Q: 1. What volume of sterile water for injection (diluent) must be added to reconstitue the powder? 2.…

A: For the 500mg vial :For the 500mg cefazolin vial, we have 2ml of diluent, and the concentration…

Q: Imagine you are a student in Alfred Hershey and Martha Chase's lab in the late 1940s. You are given…

A: Hershey and Chase experiment was just like a milestone in the history of genetics.By this experiment…

Q: Making Mammalian Cell Growth Medium 1. The cells that you are working with require the following…

A: In the question, we are given the stock solutions to prepare medium for growth of cell. From this…

Q: 1. How many mL of sterile water must be added to make the preparation isotonic? 2. How many mL of…

A: Isotonic solutions are solutions with the same concentration of solutes as the fluid inside a cell…

Q: 3. How would you use a 3-step serial dilution to produce 1ml of a luM Glucose solution from a 1M…

A: Glucose is a simple sugar that the body uses to provide energy. It is naturally present in fruits…

Q: elg äbäi An example of an antiseptic. would be A- Soap B- Rubbing alcohol O C- hand lotion O D-…

A: a liquid or cream that prevents a cut, etc. from becoming infected is called anticeptic

Q: methods of cell lysis?

A: Note: As per the guidelines, only one question has to be answered. Hence, 3.(a) will be answered as…

Q: I need hemp measurements A and B please

A: Polyacrylamide gel electrophoresis (PAGE) is a technique for separating molecules depending on their…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.