4. From all antiseptics & disinfectants tested in class which agent worked the best against Staphylococcus & Pseudomonas? What part of the cell was effected by the control method?

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4. From all antiseptics & disinfectants tested in class which agent worked the best against Staphylococcus & Pseudomonas? What part of the cell was effected by the control method? 
**Table: Inhibition Diameter of Various Agents Against Bacteria**

This table displays the inhibition diameters (in millimeters) for different agents when tested against two bacterial species: *Staphylococcus* (#2) and *Pseudomonas* (#8). The inhibition diameter reflects the effectiveness of each agent in inhibiting bacterial growth.

| Agent          | *Staphylococcus* (#2) | *Pseudomonas* (#8) |
|----------------|-----------------------|--------------------|
| 3% H2O2        | 37 mm                 | 85 mm              |
| 10% Bleach     | 40 mm                 | 54 mm              |
| 5% Lysol       | 25 mm                 | 13 mm              |
| Betadine       | 34 mm                 | 18 mm              |
| Soap           | 30 mm                 | 0 mm               |
| Scope          | 31 mm                 | 0 mm               |
| Listerine      | 0 mm                  | 0 mm               |
| Vinegar        | 55 mm                 | 28 mm              |
| Corn Syrup     | 0 mm                  | 0 mm               |
| Lemon Juice    | 35 mm                 | 13 mm              |
| 70% Alcohol    | 26 mm                 | 18 mm              |

### Observations:
- *3% H2O2* shows significant inhibitory effects on both bacteria, especially *Pseudomonas*.
- *10% Bleach* also inhibits both bacteria, though less effectively than H2O2.
- *Soap* and *Scope* are effective against *Staphylococcus* but show no effect on *Pseudomonas*.
- Substances like *Listerine* and *Corn Syrup* exhibit no inhibitory effect on either bacterium.
- *Vinegar* has one of the highest inhibition effects on *Staphylococcus*.

### Conclusion:
Different agents have varying levels of effectiveness against bacterial species. While some agents like hydrogen peroxide and bleach show broad-spectrum efficacy, others such as soap and vinegar display selective antibacterial properties.
Transcribed Image Text:**Table: Inhibition Diameter of Various Agents Against Bacteria** This table displays the inhibition diameters (in millimeters) for different agents when tested against two bacterial species: *Staphylococcus* (#2) and *Pseudomonas* (#8). The inhibition diameter reflects the effectiveness of each agent in inhibiting bacterial growth. | Agent | *Staphylococcus* (#2) | *Pseudomonas* (#8) | |----------------|-----------------------|--------------------| | 3% H2O2 | 37 mm | 85 mm | | 10% Bleach | 40 mm | 54 mm | | 5% Lysol | 25 mm | 13 mm | | Betadine | 34 mm | 18 mm | | Soap | 30 mm | 0 mm | | Scope | 31 mm | 0 mm | | Listerine | 0 mm | 0 mm | | Vinegar | 55 mm | 28 mm | | Corn Syrup | 0 mm | 0 mm | | Lemon Juice | 35 mm | 13 mm | | 70% Alcohol | 26 mm | 18 mm | ### Observations: - *3% H2O2* shows significant inhibitory effects on both bacteria, especially *Pseudomonas*. - *10% Bleach* also inhibits both bacteria, though less effectively than H2O2. - *Soap* and *Scope* are effective against *Staphylococcus* but show no effect on *Pseudomonas*. - Substances like *Listerine* and *Corn Syrup* exhibit no inhibitory effect on either bacterium. - *Vinegar* has one of the highest inhibition effects on *Staphylococcus*. ### Conclusion: Different agents have varying levels of effectiveness against bacterial species. While some agents like hydrogen peroxide and bleach show broad-spectrum efficacy, others such as soap and vinegar display selective antibacterial properties.
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In disk test, by looking at the zone of inhibition, one can say which antiseptics & disinfectants tested  worked the best against the studied microorganisms. The higher the diameter of zone of inhibition, higher is the susceptibility of the microorganisms towards that antiseptics & disinfectant.

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