Make a viable count of the donor culture by spot inoculation of he nutrient agar plate WITHOUT antibiotics as follows: Prepare a 10 fold serial dilution (to 107) of the DONOR culture in sterile saline. Show clearly how you intend to prepare this dilution series and have it checked by a demonstrator before continuing. Dilution Volume of saline (units?) Volume of which other 10-1 10-2 10-3 104 10-5 10-6 10-7 dilution? Clearly label the base of the nutrient broth agar plate as shown in
Make a viable count of the donor culture by spot inoculation of he nutrient agar plate WITHOUT antibiotics as follows: Prepare a 10 fold serial dilution (to 107) of the DONOR culture in sterile saline. Show clearly how you intend to prepare this dilution series and have it checked by a demonstrator before continuing. Dilution Volume of saline (units?) Volume of which other 10-1 10-2 10-3 104 10-5 10-6 10-7 dilution? Clearly label the base of the nutrient broth agar plate as shown in
Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter6: Basic Clinical Chemistry
Section6.3: Principles Of Chemistry Instrumentation
Problem 1CT
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Transcribed Image Text:2. Make a viable count of the donor culture by spot inoculation of
the nutrient agar plate WITHOUT antibiotics as follows:
• Prepare a 10 fold serial dilution (to 10-7) of the DONOR culture in
sterile saline.
• Show clearly how you intend to prepare this dilution series and
have it checked by a demonstrator before continuing.
Dilution
Volume of
saline (units?)
Volume of
10-1
10-2
10-3
104
10-5
10-6
10-7
which other
| dilution?
• Clearly label the base of the nutrient broth agar plate as shown in
the diagram below. Include your initials, the date, and what the
plate contains (donor viable cell count).
• Carefully dispense 3 x 10 µl drops of the 104, 10-5, 106 and 10-7
dilutions of the culture within quarter areas of the agar plate then
replace the lid. Allow the inocula to soak into the agar before
inverting the plate for incubation.
• What is the purpose of this step?
49
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