Induction experiment Results Table 2: Culture growth Sample no Time Oh 1h 2h 3h 4h 12.02 13.02 10 Dilution factor OD600 Measured 14.02 10 15.03 16.02 20 20 0.809 0.150 0.287 0.206 0.249 Corrected OD (OD 600 Measured * dilution factor) 0.809 1.5 2.87 4.12 4.98 N) Draw the growth curve on the semi logarithmic paper (add a picture in the report). Remember that bacterial growth is exponential, so use semi logarithmic paper to plot OD 600 as a function of time. O) Comment on the growth curve, is the bacteria growing exponential? P) Identify the interval in which the growth is exponential and use the interval to identify the doubling time of the culture. What is the mass doubling time of the culture during exponential growth (in minutes)?

Induction experiment Results Table 2: Culture growth Sample no Time Oh 1h 2h 3h 4h 12.02 13.02 10 Dilution factor OD600 Measured 14.02 10 15.03 16.02 20 20 0.809 0.150 0.287 0.206 0.249 Corrected OD (OD 600 Measured * dilution factor) 0.809 1.5 2.87 4.12 4.98 N) Draw the growth curve on the semi logarithmic paper (add a picture in the report). Remember that bacterial growth is exponential, so use semi logarithmic paper to plot OD 600 as a function of time. O) Comment on the growth curve, is the bacteria growing exponential? P) Identify the interval in which the growth is exponential and use the interval to identify the doubling time of the culture. What is the mass doubling time of the culture during exponential growth (in minutes)?

Basic Clinical Lab Competencies for Respiratory Care: An Integrated Approach

5th Edition

ISBN:9781285244662

Author:White

Publisher:White

Chapter1: Basics Of Asepsis

Section: Chapter Questions

Problem 8SEPT

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