Induction experiment Results Table 2: Culture growth Sample no Time Oh 1h 2h 3h 4h 12.02 13.02 10 Dilution factor OD600 Measured 14.02 10 15.03 16.02 20 20 0.809 0.150 0.287 0.206 0.249 Corrected OD (OD 600 Measured * dilution factor) 0.809 1.5 2.87 4.12 4.98 N) Draw the growth curve on the semi logarithmic paper (add a picture in the report). Remember that bacterial growth is exponential, so use semi logarithmic paper to plot OD 600 as a function of time. O) Comment on the growth curve, is the bacteria growing exponential? P) Identify the interval in which the growth is exponential and use the interval to identify the doubling time of the culture. What is the mass doubling time of the culture during exponential growth (in minutes)?
Induction experiment Results Table 2: Culture growth Sample no Time Oh 1h 2h 3h 4h 12.02 13.02 10 Dilution factor OD600 Measured 14.02 10 15.03 16.02 20 20 0.809 0.150 0.287 0.206 0.249 Corrected OD (OD 600 Measured * dilution factor) 0.809 1.5 2.87 4.12 4.98 N) Draw the growth curve on the semi logarithmic paper (add a picture in the report). Remember that bacterial growth is exponential, so use semi logarithmic paper to plot OD 600 as a function of time. O) Comment on the growth curve, is the bacteria growing exponential? P) Identify the interval in which the growth is exponential and use the interval to identify the doubling time of the culture. What is the mass doubling time of the culture during exponential growth (in minutes)?
Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Control of Microbial Growth
It can be defined as the process to inhibit or prevent the growth of the population of microorganisms. It usually involves the use of physical and chemical agents to the growth of microorganisms. It is very important to control the growth of microorganisms, especially in the pharmaceutical and biotechnology industries, academic research, medical field, and food industry.
Sterilization
It is a method for the destruction of all forms of vegetative growth and endospores from a medium. In other words, sterilization can be defined as a method of killing every microbial organism such as bacteria, fungi, etc. present in an object or a medium. There are different strategies present to sterilize an object, like physical methods and chemical methods of microbial control.
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GROWTH CUVRVE
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(This is a question from GFP experiment I did, which I have to include in my rapport and hand in)
Transcribed Image Text:Induction experiment
Results Table 2: Culture growth
Sample no Time
Oh
1h
2h
3h
4h
12.02
13.02
14.02
15.03
16.02
Dilution factor OD600 Measured Corrected OD
10
10
20
20
0.809
0.150
0.287
0.206
0.249
(OD 600M
0.809
1.5
2.87
4.12
Measured * dilution factor)
4.98
N) Draw the growth curve on the semi logarithmic paper (add a picture in the report).
Remember that bacterial growth is exponential, so use semi logarithmic paper to plot OD 600
as a function of time.
O) Comment on the growth curve, is the bacteria growing exponential?
P) Identify the interval in which the growth is exponential and use the interval to identify the
doubling time of the culture. What is the mass doubling time of the culture during
exponential growth (in minutes)?
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