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Why would gel filtration chromatography be the best method for purifying an antigen for injection into a mouse to generate monoclonal antibodies? Antigens can easily be purified by charge and size when coated with SDS O Antigens will separate by size going through the column beads and their holes Antigens can be centrifuged through a column and pellet at specific speeds O Organelles containing the antigen will have a specific buoyancy and separate in a gradient Antibodies can be used to detect the antigen on a western blot

Why would gel filtration chromatography be the best method for purifying an antigen for injection into a mouse to generate monoclonal antibodies? Antigens can easily be purified by charge and size when coated with SDS O Antigens will separate by size going through the column beads and their holes Antigens can be centrifuged through a column and pellet at specific speeds O Organelles containing the antigen will have a specific buoyancy and separate in a gradient Antibodies can be used to detect the antigen on a western blot

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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**Question:** Why would gel filtration chromatography be the best method for purifying an antigen for injection into a mouse to generate monoclonal antibodies? **Options:** - Antigens can easily be purified by charge and size when coated with SDS - Antigens will separate by size going through the column beads and their holes - Antigens can be centrifuged through a column and pellet at specific speeds - Organelles containing the antigen will have a specific buoyancy and separate in a gradient - Antibodies can be used to detect the antigen on a western blot **Explanation:** Gel filtration chromatography is a technique where molecules are separated based on their size as they pass through a column filled with porous beads. Smaller molecules enter the beads and take longer to elute, while larger molecules bypass the beads and elute faster. This method is particularly useful for purifying antigens due to its ability to separate them solely based on size.
Transcribed Image Text:**Question:** Why would gel filtration chromatography be the best method for purifying an antigen for injection into a mouse to generate monoclonal antibodies? **Options:** - Antigens can easily be purified by charge and size when coated with SDS - Antigens will separate by size going through the column beads and their holes - Antigens can be centrifuged through a column and pellet at specific speeds - Organelles containing the antigen will have a specific buoyancy and separate in a gradient - Antibodies can be used to detect the antigen on a western blot **Explanation:** Gel filtration chromatography is a technique where molecules are separated based on their size as they pass through a column filled with porous beads. Smaller molecules enter the beads and take longer to elute, while larger molecules bypass the beads and elute faster. This method is particularly useful for purifying antigens due to its ability to separate them solely based on size.
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Biotechnology is a branch of biology, including the use of living organisms to produce products. Based on the instruments and applications, it usually overlaps with relevant scientific disciplines. Biotechnology includes different sciences, such as genomics, recombinant gene technology, applied immunology, and the development of pharmaceutical medicines and diagnostic experiments.

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