ne assay. When cyclin D1 activates Cdk4 and Cdk6, the activated Cdks phorylate retinoblastima protein (RB). Purified V was incubated with purified -, purified RB protein, and radiolabeled ATP. As a control, purified Cdk6, purifiec rotein, and radiolabeled ATP were incubated without purified V. One hour later, the enzyme assay mixes were separated by PAGE. The PAGE gel was exposed to X-ray film. The developed film is shown below. V/cdk6 What is the purpose of the control shown at left?
ne assay. When cyclin D1 activates Cdk4 and Cdk6, the activated Cdks phorylate retinoblastima protein (RB). Purified V was incubated with purified -, purified RB protein, and radiolabeled ATP. As a control, purified Cdk6, purifiec rotein, and radiolabeled ATP were incubated without purified V. One hour later, the enzyme assay mixes were separated by PAGE. The PAGE gel was exposed to X-ray film. The developed film is shown below. V/cdk6 What is the purpose of the control shown at left?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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AaBbCcl AaBbCcD
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3) Enzyme assay. When cyclin D1 activates Cdk4 and Cdk6, the activated Cdks
phosphorylate retinoblastima protein (RB). Purified V was incubated with purified
Cdk6, purified RB protein, and radiolabeled ATP. As a control, purified Cdk6, purified
RB protein, and radiolabeled ATP were incubated without purified V. One hour later,
the enzyme assay mixes were separated by PAGE. The PAGE gel was
exposed to X-ray film. The developed film is shown below.
V/cdk6.
What is the purpose of the control shown at left?
What do the results on the right demonstrate?
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