You were given a mixed nutrient agar broth culture of bacteria  1a. How will you determine the different types of bacteria present in the mixed culture without using an agar plate 1b. How will you make a pure culture of these bacteria on a slant nutrient agar 1c. How will you identify these bacteria from the pure culture without using an agar plate

Q: Calculate the CFU/ml of leuconostoc in the brine for the 4 different days. Show your calculation

A: CFU/ml stands for "colony forming units per milliliter." It is a unit of measurement used in…

Q: student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step…

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Q: Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL…

A: Serial dilution is a progression of successive dilutions used to lessen a thick culture of cells to…

Q: A culture of E. coli has a concentration of 5 x 108 cells/mL. How many times do you have to dilute…

A: Given: 1. Number of colonies: 2502. No. of bacteria in culture: 5 X 108 3. Volume plated: 0.1 ml 4.…

Q: how many coliform colonies would you expect to see on your 1:10 plate? Your 1:100 plate? Explain.

A: Introduction Coliform is the gram-negative, non-spore-forming and motile or non-motile bacterial…

Q: You make a 1:100 dilution of your culture and plated 0.1 ml of this sample on a Nutrient agar plate.…

A: The amount of organisms present in the original culture is called as colony forming unit per…

Q: What is the correct shape of the unknown bacterium growing on your MacConkey agar?

A: Macconkey Agar This agar includes bile salts, which are found in the gut. Bile salts help in…

Q: This agar plate was obtained by spreading 0.1 ml of a 1:1,000 dilution of a bacterial sample taken…

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Q: A sample of water was enumerated using a counting chamber (haemocytometer) and 220 bacterial cells…

A: The viable plate count, or simply plate count, is a count of viable or live cells. It is based on…

Q: Describe how you would execute this first stage from the point you are handed the nutrient broth…

A: For isolation of any culture, it needs to be incubated in broth to obtain full fledged turbidity or…

Q: 1) What are all of the different colony types that can be seen on a mixed-culture streak plate?  2)…

A: Hi! Thanks for your question. As you have posted multiple questions, I am answering only the first…

Q: Which type(s) of medium would be used in each scenario?a. isolating the growth of Streptococcus…

A: A culture medium, also called the growth medium, can be a liquid or solid medium that supports the…

Q: Remove the agar plate from its lid with the left hand. While holding the plate, inoculate the agar…

A: Introduction -- Microbes are around and every where in environment. Sterile agar plates which…

Q: If you were using the quadrant streak plate method to plate a very dilute broth culture ( with many…

A: Quadrant streaking is a microbiological technique which is used for isolation of pure strain from…

Q: What is an E-test strip, and what is it used for in the microbiology laboratory? Draw a diagram of…

A: Microbiology is a branch of science which deals with the study of microscopic organisms such as…

Q: A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution…

A: A sample of your blood, urine, skin, or any part of your body will be taken during a bacteria…

Q: You are transferring a bacterial culture from a tube of broth to a Petri dish containing trypticase…

A: Bacterial culture: It is a technique that enables the controlled multiplication of bacterial cells…

Q: How do eosin-methylene blue (EMB) agar plates work? What organism(s) are they designed to detect?…

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Q: One hundred microliters (0.100 ml) of a 10-5 dilution of a bacterial suspension plated on an agar…

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Q: Out of the three gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, and…

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Q: Please explain how the use of an Eosin Methylene Blue Agar plate can help determine the type of…

A: Eosin Methylene Blue (EMB) agar is a differential microbiological medium, which somewhat hinders the…

Q: which of these tests (i.e gram stain, acid fast stain, endospore, oxidation to fermentation,…

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Q: What would be the expected outcome of an endospore stain test If the bacteria is a pathogenic…

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Q: Y ΝΑ XIW Hfr NA Nutrient Agar Provides all nutrients the bacteria needs + no antibiotics y ECM XIW…

A: The ECM agar is composed of the minimum nutrients required for the growth of bacterial cells,…

Q: EMB and MSA results, build a dichotomous key that could differentiate between these four strains.

A: A dichotomous key is a representation of precise characteristics of organisms for the ease of their…

Q: you are given a mixed culture of s. aureus, E. coli and P. aeruginosa. how would you isolate each…

A: In the study of microorganisms, a mixed culture is one that contains more than one type of organism…

Q: How does the agar dilution method differ from streaking toobtain isolated colonies?

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Q: Based on the growth you saw on your Lee’s Agar plate, what conclusions can you make about the…

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Q: Which isolation technique is best used in isolating microorganisms (in countable good plates) from a…

A: The phrase "isolation" in microbiology refers to the separation of a strain from a natural, mixed…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.