4. TLC is still useful in the lab but is being replaced by automatedchromatographic methods. For example, TLC, FPLC, and HPLCare now automated processes, and computers can control variousparameters, such as flow rate, blends of solvent mixtures, etc.Can you think of how the ability to manipulate the parameters ofthese automated chromatographic methods (sample size, columnplate length, flow rate, solvent gradients, etc.) might accentuate orimprove an investigator's ability to separate amino acids or otherbiomolecules?

Answered: 4. TLC is still useful in the lab but…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

4. TLC is still useful in the lab but is being replaced by automated
chromatographic methods. For example, TLC, FPLC, and HPLC
are now automated processes, and computers can control various
parameters, such as flow rate, blends of solvent mixtures, etc.
Can you think of how the ability to manipulate the parameters of
these automated chromatographic methods (sample size, column
plate length, flow rate, solvent gradients, etc.) might accentuate or
improve an investigator's ability to separate amino acids or other
biomolecules?

Expert Solution

Step 1

Automated chromatography is a process in which the separation of molecules is carried out automatically by a computer-controlled system.
The system can be programmed to adjust various parameters such as flow rate, solvent mixtures, column temperature, and detection parameters to optimize the separation of specific compounds or biomolecules.

There are several types of automated chromatographic methods, including: