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- 6. Answer the following questions about colony forming units. a. b. You take a culture of bacteria and dilute it 5 times. Each dilution uses 1 ml of culture and 9 ml of saline solution. You take .1ml from the most dilute tube and plate it on a TSA plate. The next day you count 61 colonies. What was the original concentration a) 6.1 X 105 CFU/ml b) 6.1 X 105 CFU/ml c) 6.1 X 108 CFU/ml d) 6.1 X 107 CFU/ml You are doing a series of serial dilutions to find the concentration of bacteria in CFU/ml in a culture. You plate several of the dilutions tubes and count the colonies the next day. On plate A you find over 1000 colonies, plate B you find 157 colonies and plate C you find 7 colonies. Which plate(s) can be used to determine the concentration in the original tube? a) Plate A Ob) None of the plates can be used c) Plate C d) Plate B e) Plates A, B and CA. Microscopic Observation of Cells Wet Mounts, Gram stain and spore stain 1 Describe the microscopic morphology and behavior of each species observed with a wet mount (A. serpens and E. gracilis). 2 Describe the microscopic morphology of each sample observed with a Gram stain (S. aureus, P. aeruginosa, B. subtilis and dental plaque). Indicate the Gram reaction of each. 3Describe the microscopic morphology of each sample observed with the spore stain (P. aeruginosa and B. subtilis). In which of the sample can you see spores?2. You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A. Bacteria growing on the surface. B. Bacteria growing throughout the tube, the agar shows cracks. C. Bacteria growing about 5 mm below the surface. Please interpret each bacterial result. (Give the bacteria an oxygen classification, explain what classification means and interpret the cracks in the agar.) 3. Please explain how the use of an Eosin Methylene Blue Agar plate can help determine the type of fermenters that bacteria are. Please explain thoroughly.
- I U Cardo 12 1三 ニ 三 rI TI3 4. 111 I. 5. What are the basic shapes of bacterial cells? What are the basic Know the terms. Which types of organisms have axial filaments? How would classify the following in terms of gram stain reaction and shape and arrangement of the cells Escherichia coli? Staphylococcus aureus? Streptococcus pyogenes? Bacillus anthracis? Do these organisms cause any important diseases? arrangements? you III1:07 < Вack Untitled 6 Brownian movement of bacteria: O a. Can be observed by the of soft agar technique O b. Can be observed by staining of Staphylococcus aureus Oc. Is common in Escherichia coli or Proteus vulgaris O d. Is common in Staphylococcus aureus O e. Can be observed at the edge of a microscope CLEAR MY CHOICEExplain the statement below: 1. Animal cell culture makes use of low amounts of reagents.
- 1. How could pastes be used to treat wounds? 2. Explain why a paste provides more of an occlusive effect than a lotion 3. Are pastes biphasic or single-phase? Explain.1. If you expose E.coli to UV light at 30 seconds, 1 minute, 3 minutes and 5 minutes, what are your expected results? 2. If you expose. B. cereus to UV light at 30 seconds, 1 minute, 3 minutes and 5 minutes, what are your expected results? 3. If you do not remove the cover of the plate before exposure to UV light at 5 minutes for E.coli and B. cereus, what are your expected results? Help with 1,2 & 3 please8. Pigment or color (e.g.: opaque, translucid, red, yellow, rose, violet, etc..) is also another characteristic that can be used to identify your microorganism in a slant Thus, write down the name of three microorganisms (using scientific notation: Escherichia coli) that present a pigmented pattern of growth in a slant (write down the color of each pigment) and the name of the disease that they may cause. F10 F12 F6 %24 6. R. Home Enter K F G A Shift C Page Up Page Down Alt Ctrl Alt Σ
- 1 H 2 176 old culture Smear too BOTERC Losk like? + b. Failure to apply the decolorizer. + ne c. Failure to apply the safranin. Determine whether bacteria are motle or non-motle by looking at the growth in semi-solid medium What is the concentration of agar in (a) typical solid medium (b) semi sold motility medium? Arawer Q1 on Page 357. 7. Orygen Requirement: + d. Reversal of crystal violet and safranin stains. A questions 1.2 and 3 o Pg 83-84 Refer to the power point Questions 1 Predict the effect of the following "mistakes" made when performing a Gram stain. Your answer needs to address the predicted effect on both Gram-positive and Gram-negative cells. Consider each mistake independently. a. Failure to add the iodine. 414 gait to fer. 2 Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram-positive and Gram-negative cells. This being the case, explain how they stain different cell types in the Gram stain. why? a cell wall. patem in…1.What types of damage to human tissues can result from prolonged exposures to UV radiation? What protective measures can be taken to limit these types of damage, both during the experiment and in everyday life? 2.Which B. megaterium culture, logarithmic or stationary phase, would show the best survival following exposure to UV radiation?Pregunta 7 Diluting a culture of bacteria to detemine the cancentration OC) Generally not required for well-established E. ull cultures O (D) Both A and B are correct O A) Performed on a liquid bacterial culture to determine the type of organism present O (B) Involves the use of spread plating Pregunta 8 Preparing a cell extract O (B) May involve the use of a detergent O (D) A-C are correct OWs done to prepare a sample for chromatography O (C) May involve the use of an enzyme Pregunta 9 Preparing a cell extract as we did in class O A Resulted in a cell extract with all denatured proteins pok O (C) Both B and D are correct O (B) Involved SDS orm oring O (D) Resulted in a cell extract that maintalned protein tertiary structures Teams