While measuring the turbidity of a bacterial culture in class, Kevin recorded both the absorbance and transmission values of his sample. While working on his lab report, Kevin is unsure of which value to report as the optical density. His lab partner tells him that he should use transmission. Is this correct? True False

Q: Peptidoglycan on bacterial cell walls is stained by crystal violet dye and for this reason those…

A: True

Q: Give two reasons as to why anyone would bother SYBRGreen (SG) staining samples that contain…

A: SYBR Green (SG) is an asymmetrical cyanine nucleic acid stain. It binds to DNA.

Q: Which disinfectant is the most effective? and why? Which disinfectant(s) is (are) bactericidal? and…

A: Disinfectant A is most effective as  there is no growth of salmonella choleraesuis at dilution 1:2…

Q: If you perform the motility test on Motility Medium and hanging drop for Bacillus cereus and B.…

A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…

Q: As part of an experiment where absorbance values are measured using a spectrophotometer, you are…

A: The bacteria growth uses mitotic cell divisions. The bacterial population grows until the nutrients…

Q: What are the disadvantages of doing a total microbial count by examining wet mounts under the…

A: Ans- Wet mounts have the following drawbacks: The benefit of being able to observe movement can also…

Q: Agar is a solidifying agent used in media preparation. What is its origin?

A: Agar gel is a polysaccharide which can become liquefied at temperatures ranges from 85°C to 90°C and…

Q: Bacillus subtilis (Gram-positive) and Pseudomonas aeroginosa (Gram-negative) bacteria are heat-fixed…

A: Bacteria have cell walls made up of polysaccharides that give them strength.Bacterial cell walls…

Q: What is the physical appearance (solid, liquid, semi-solid) of following media: agar plate

A: The Agar plate is made from mixing agarose and a growth medium onto a petri dish. Agarose is in a…

Q: Collecting data that can then be transformed into a bacterial growth curve is usually done using a…

A: A spectrophotometer is an instrument used to measure the amount of light absorbed by a sample. The…

Q: identify the organisms in  an unknown culture. You find that the culture contains two gramnegative…

A: A culture is a medium to grow bacteria and other microorganisms  It provides the bacteria with…

Q: If you run the motility test on Motility Medium and hanging drop for Bacillus cereus and B.…

A: Answer:  HANGING DROP TECHNIQUE : It is the special type of wet mount which is made from a drop of…

Q: Jessie is working with a 100% stock concentration of Clorox. She decides to add 100 μL of bacteria…

A: For calculating the unknown concentration of the tube we will be using the formula as mentioned…

Q: For a laboratory exercise of basic techniques in microbiology: Gram stain and Microscopy. What are…

A: "Gram staining is important in microbiology because it allows scientists to quickly and easily…

Q: If each agar plate contained 20 ml of LB agar and had an ampicillin concentration of 150 ug/ml, what…

A: Given data:  Each plate contains-  LB agar=20ml Ampicillin= 150 micrograms per ml.  Amount of…

Q: You have 0.05 ml of kombucha that contains microbes at a concentration of 8.2 x 10^6 CFU/ml. You…

A: Creating proper dilutions and working out the colony-forming units or CFU/ ml is one integral part…

Q: For a laboratory exercise of basic techniques in microbiology: Gram stain and Microscopy. What are…

A: Gram staining procedure begins with preparing of a smear of bacterial culture. This  step is called…

Q: one or your lab partners has followed the rrecommended procedure of running Gram-positive and Gram…

A: Gram staining is a common laboratory test to characterize the bacteria into gram-positive and…

Q: chemical(s) best at inhibiting both Gram- negative and Gram-positive bacteria [Choose ] [Choose ]…

A: Hi! Thanks for your question. As you have posted multiple questions and have not mentioned which one…

Q: chemical(s) best at inhibiting both Gram- negative and Gram-positive bacteria [ Choose ] chemical…

A: Bacteria are microscopic, unicellular, prokaryotic organisms found in diverse environments. They can…

Q: One of your lab partners has followed the recommended procedure of running Gram-positive and…

A: A popular method used to separate two large groups of bacteria, depending on the different…

Q: Why do we use the term CFU (colony forming unit) instead of reporting bacteria per ml? Explain.

A: The  bacterial species are responsible for causing several diseases within the human body. So, for…

Q: Which of the following can be used to distinguish one colony from another on an agar plate?  Select…

A: A colony is a visible cluster of microorganisms like fungi and bacteria that grow on a solid growth…

Q: Using absorbance readings and a standard curve relating absorbance to cell number, you suspect that…

A: The countable range of colonies for an ideal plate count method is considered 30-300 colonies per…

Q: Unlike a rabbit, running all-out for a few moments to escape a predator, migratory birds require…

A: Acetyl coenzyme A is a key metabolic intermediate that is produced in a variety of methods,…

Q: Describe characteristics of Streptococcus Agalactiae in the Agar: (How does colonies look like…

A: Streptococcus agalactiae is a Gram-positive, cocci-shaped bacteria that typically form chains. It is…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.