One of your lab partners has followed the recommended procedure of running Gram-positive and Gram-negativecontrol organisms on her Gram stain of an unknown species. Her choices of controls were Escherichia coli andBacillus subtilis. She tries several times and each time concludes she is decolorizing too long because bothcontrols have pink cells (one more than the other). What might you suggest she try and why?

Answered: One of your lab partners has followed…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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One of your lab partners has followed the recommended procedure of running Gram-positive and Gram-negative
control organisms on her Gram stain of an unknown species. Her choices of controls were Escherichia coli and
Bacillus subtilis. She tries several times and each time concludes she is decolorizing too long because both
controls have pink cells (one more than the other). What might you suggest she try and why?

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A popular method used to separate two large groups of bacteria, depending on the different components of the cell wall, is called gram staining. A thick cell wall consisting of a protein called peptidoglycan is the characteristic feature of gram-positive bacteria. Gram-negative bacteria, on the other hand, have a delicate layer of peptidoglycan situated between an internal cell membrane and an external membrane. By rendering these gram-negative and gram-positive bacterial cells pink or violet, respectively, the Gram stain technique differentiates between gram-positive and gram-negative types.

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