A colleague performs a smear prep from a colony on an agar plate. Without staining it, he looks at it under oil immersion with a light microscope (1000x magnification). “Hm," he says, "it's not purple, so it must be gram negative." Briefly explain the error in his logic, and say what he would have to do to show whether the organism is gram negative or gram positive. Your Answer:

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Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.