Imagine that you are Gram staining a mixed culture of both gram-positive and gram-negative bacteria. If you omitted the iodine (mordant) step in the procedure, what colors would the bacteria on your slide appear to be?

Q: You look at a slide you prepared and, despite the fact that you know you put organism onto the slide…

A: Gram-staining is a laboratory test that detects the presence of bacteria and also helps to…

Q: What is gram staining and its purpose? Why is it only applicable in bacteria?

A: In the differential staining method, various stains are applied. These methods can be utilized for…

Q: Why are basic dyes more effective for bacterial staining than acidic dyes?

A: Staining is a technique used in slide preparation to colour the specimen.      With light microscopy…

Q: Why is the Gram stain considered a differential stain?

A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…

Q: An acid-fast staining method was performed on a bacterial smear using the dyes carbolfuchsin and…

A: Introduction: Acid-fast staining is a type of differential staining technique that was first…

Q: hy is Gram staining so important for bacterial characterization (i.e. what o )? In one paragraph,…

A: Gram staining is a very important criteria for distinguishing certain kinds of bacteria into two…

Q: Please discuss why staining is important in visualizing microbes under the microscope. What…

A: Staining is an essential step in visualising microbes under the microscope. THE NECESSITY OF…

Q: What are the pros and cons of using the TCE stain-free method versus a traditional gel staining…

A: PAGE (polyacrylamide gel electrophoresis) is a well-known method for separating, detecting, and…

Q: Bacillus subtilis (Gram-positive) and Pseudomonas aeroginosa (Gram-negative) bacteria are heat-fixed…

A: Bacteria have cell walls made up of polysaccharides that give them strength.Bacterial cell walls…

Q: Move the images of bacterial cells into the chart to illustrate the color of gram-positive and…

A: Gram staining is a technique used to differentiate between two groups of bacteria namely…

Q: What is the purpose of acid-fast staining? What information can you gain from this procedure? Please…

A: The acid-fast stain is used to identify acid-fast organisms. Acid-fast organisms posess nearly…

Q: Why is oil immersion necessary to see bacteria in the laboratory?

A: Oil immersion technique is used to increase the resolving power of microscope. Both the objective…

Q: In four steps, describe the simple staining procedure.

A: A stain is used to clearly view a specimen under the microscope. In simple staining, bacteria are…

Q: Examples of bacteria observe in the microscope using Gram stain, Ziehl-Neelsen, and Kinyoun Method.

A: Ziehl–Neelsen staining is a type of acid-fast stain, first introduced by Paul Ehrlich. Ziehl–Neelsen…

Q: Jessica placed crystal violet ,grama's iodine and safranin reagents on her bench.Which staining…

A: The correct option is . A Gram's Staining procedure

Q: Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?

A: In microscopic studies, the specimen needs to be prepared for an optimized vision under a…

Q: Describe one error in the staining procedure that would result in a Gram-positive bacterium…

A: Gram staining is a differential staining technique that differentiates between a gram-positive and a…

Q: Choose the dyes or reagents used in the Endospore stain. (More than one choices are correct)   a…

A: Simple stains- A stain that employs a single stain to color a bacteria: they are quick and easy.…

Q: Create paragraphs to differentiate among the following terms: positive stain, negative stain, simple…

A: An organic molecule with a benzene ring, a chromophore, and an auxochrome group is known as a…

Q: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…

A: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…

Q: Is acid fast staining differential stain , if yes, what are the 2 types of stain used

A: Differential Staining is defined as a staining process which utilizes more than one chemical stain.…

Q: Is is best to observe slides of bacteria stained with the acid fast stain under high dry…

A: The acid-fast stain is used to stain the acid-fast organisms. Acid-fast microorganisms have a thick…

Q: The acid-fast stain is used to stain _____. The decolorizer used to destain the cells is ______.…

A: The staining procedures used to differentiate the types of bacteria are known as a differential…

Q: What step normally associated with staining bacterial cells is omitted when the dimensions of cells…

A: Step 1 Gram staining is a technique of staining that is used to differentiate and categorize…

Q: If you prepared a sample of a specimen for light microscopy, stained it with the Gram stain, and…

A: Bacteria are unicellular prokaryotic microorganisms (nucleus is not enclosed in a nuclear membrane)…

Q: microscope

A: Microscope helps to see the small things clearly

Q: list the major steps, in sequence needed to perform the schaeffer-fulton and crystal violet…

A: Endospores are structures produced within certain bacterial cells that allow them to survive harsh…

Q: Based from the results obtained from Gram staining, explain why bacterial cells retained or changed…

A: Staining is a technique that is used to enhance the contrast of the microscopic image through the…

Q: You mistakenly confused the primary stain and counter stain with crystal violet, how does you…

A: Gram staining is a widely used laboratory technique in microbiology and bacteriology to…

Q: You and a partner conduct the acid-fast staining using the same two bacteria. You know that there…

A: Acid-fast staining is a differential staining technique that proves to be an important diagnostic…

Q: Gram-positive (+) bacteria with a thin peptidoglycan layer traps the crystal violet stain. Select…

A: Gram staining is also known as Gram's method is a staining method for identifying and separating…

Q: Out of the three gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, and…

A: Bacteria are small, microscopic and unicellular organisms that can live in variety of environment.…

Q: How do Blood agar & EMB agar help us to identify organisms more specifically than a gram stain?

A: How do Blood agar & EMB agar help us to identify organisms more specifically than a gram stain?

Q: Name a few group of microorganisms where Grams staining will not work and/or not appropriate to use.…

A: In Gram staining crystal violet (primary stain). stain is used to color the bacterial cell wall. The…

Q: Why is it essential that smears be air-dried?  Why can’t they be gently heated over a flame to speed…

A: Introduction :  A bacterial smear is a tiny amount of culture that has been applied to the slide's…

Q: List the primary stain, secondary stain, mordant, and decolorizer for the gram stair and write them…

A: The strains in the plate is give on two strains the primary strain and secondary strain. The primary…

Q: After performing the gram stain you observe your result as gram negative. To help confirm this…

A: Gram staining is a technique to identify bacterial stain gram-positive or gram-negative. It can be…

Q: what effect would failing to drain the smear in-between staining have on your judgment of the gram…

A: Gram staining is a technique used to differentiate between two groups of bacteria namely…