Can I get help on finding the appropriate amount? Materials Provided per Group of 4 studentsOne 10 cm dish of 80% Confluent A549 cellsOne sterile 10 cm cell culture plate5 ml and 10 ml sterile serological pipettesSterile Pasteur pipettesSterile ddH2O20 ml sterile FBS20 ml sterile 100 mM glucose20 ml sterile 200 mM glutamine20 ml sterile 100 mM pyruvate20 ml sterile 83 g/L DMEM20 ml sterile 37 g/L NaHCO3Sterile 250 ml glass bottle100 ml sterile 1X PBS20 ml sterile TrypLEOne sterile 15 ml conical tube Making Mammalian Cell Growth Medium1. The cells that you are working with require the following constituents in the growth medium:10 mM glucose4 mM glutamine1 mM pyruvate8.3 g/L DMEM3.7 g/L NaHCOз10% FBS2. Take time to calculate the appropriate amount of each of the above items to prepare 100 ml of growthmedium using the prepared liquid stock reagents. ***Check with your laboratory instructor beforeproceeding.

Answered: Making Mammalian Cell Growth Medium 1.…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Can I get help on finding the appropriate amount?

Materials Provided per Group of 4 students
One 10 cm dish of 80% Confluent A549 cells
One sterile 10 cm cell culture plate
5 ml and 10 ml sterile serological pipettes
Sterile Pasteur pipettes
Sterile ddH2O
20 ml sterile FBS
20 ml sterile 100 mM glucose
20 ml sterile 200 mM glutamine
20 ml sterile 100 mM pyruvate
20 ml sterile 83 g/L DMEM
20 ml sterile 37 g/L NaHCO3
Sterile 250 ml glass bottle
100 ml sterile 1X PBS
20 ml sterile TrypLE
One sterile 15 ml conical tube

Making Mammalian Cell Growth Medium
1. The cells that you are working with require the following constituents in the growth medium:
10 mM glucose
4 mM glutamine
1 mM pyruvate
8.3 g/L DMEM
3.7 g/L NaHCOз
10% FBS
2. Take time to calculate the appropriate amount of each of the above items to prepare 100 ml of growth
medium using the prepared liquid stock reagents. ***Check with your laboratory instructor before
proceeding.

Expert Solution

Step 1

In the question, we are given the stock solutions to prepare medium for growth of cell. From this stock solution, we have to determine the volume to be taken from the stock solution so that we can get the desired concentration of components required to make growth medium.

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