Red blood cells are dropped in a solution of unknown concentration. Looking through the test tube, the image behind is cloudy. Upon microscopic examination we see that the cells seem to have shriveled up. What does this tell us about the solution? O It is isotonic O It is hypotonic It is hypertonic
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- When placed in a hypotonic solution, a red blood cell will undergo O Hemolysis Plasmolysis Crenation Turgidity None of the aboveRefer to the following diagram: 100 ul 100 uL 100 pl 100 µL 100 pl 100 pL 100 pl Volume to transfer A B C D E F G 900 uL in each tube 10 102 10 10 10 10 10 Dilutions E.CoN culture Volume of the diluted sample plated 100 ul 100 μL 100 pL 100 ul Volume of the 10 ml 10 ml original sample plated 10 ml 10 ml Plate D Plate E Plate F Plate G Given that Plate D has too many colonies to count, and Plate G has no colonies at all as a result of serial dilution, draw out all four plates and demonstrate what they might look like (assuming serial dilution has been performed correctly). Based on your drawings, identify the number of CFU/ml for Plate E and Plate F. Show your calculations.Anna is training to be a cell culture technician. She uses some sterile distilled water to wasg a batch of cell culture plates. When she looks at the cell culture plates under the microscope to check the cells after this, she notices the cell have burst. She realizes she should have used 0.9% saline instead. Explain what has happened, and why she should have used the saline.
- Below are the results of our disc diffusion study using antiseptics and disinfectants. Use the results to answer the following questions: Lab Lab Lysol 1:10 diluted Disinfectant Isopropanol soap Tincture bleach of iodine ISP T of I BL DIS SO LYS Diameter in mm of zone of inhibition E.coli 40 mm 20 mm 10 mm 15 60 mm Bacillus 50 mm 35 mm 15 mm 8 70 mm cereus 201. How many mL of sterile water must be added to make the preparation isotonic? 2. How many mL of another isotonic solution must be added to make the final volume of the solution?6. Answer the following questions about colony forming units. a. b. You take a culture of bacteria and dilute it 5 times. Each dilution uses 1 ml of culture and 9 ml of saline solution. You take .1ml from the most dilute tube and plate it on a TSA plate. The next day you count 61 colonies. What was the original concentration a) 6.1 X 105 CFU/ml b) 6.1 X 105 CFU/ml c) 6.1 X 108 CFU/ml d) 6.1 X 107 CFU/ml You are doing a series of serial dilutions to find the concentration of bacteria in CFU/ml in a culture. You plate several of the dilutions tubes and count the colonies the next day. On plate A you find over 1000 colonies, plate B you find 157 colonies and plate C you find 7 colonies. Which plate(s) can be used to determine the concentration in the original tube? a) Plate A Ob) None of the plates can be used c) Plate C d) Plate B e) Plates A, B and C
- Steven is a lab research assistant. He mixed 25 µL of cells with 50 µL of cell culture media in tube-A, then transferred 10 µL to the hemocytometer and started counting cells, then realized the cells were too crowded to count accurately, so Steven took an aliquot of 20 µL cells of cells from tube-A and mixed with 180 µL of cell culture media, transferred 10 µL onto the hemocytometer, and countered 500 cells from the squares on the four corners of the hemacytometer. What is the # of cells per mL? Please show all calculation steps.A dialysis bag that contains 20% sucrose solution is placed in a beaker with a 5% sucrose solution. Which solution is hypotonic? cannot be determined both solutions the 20% sucrose solution the 5% sucrose solutionThe goal of Disc diffusion test is Select one: to determine the suitable concentration of an antibiotic To identify the type of the bacteria all choices are correct to choose a suitable antibiotic
- You plate 100 uL of a bacterial culture on an LBA plate and incubate for one day at 37 degrees Celsius. You count 100 colonies (colony forming units). What is the concentration of cells you plated (represented in units of CFU/mL)? Hint: look at the units carefully. I put 10000 last time and it was wrong!! that is all the information given... 5 cfu/mL b. 1,000 cfu/mL c. 500 cfu/mL d. 1 cfu/mL e. 10,000 cfu/mLDescribe how you would prepare a dilution series of a 1 x 107 CFU/mL culture to the 10-8 dilution using only 4.5 mL diluents in tubes. What would be the theoretical cell count if 0.1 mL of the 2nd and 4th dilutions were each plated?A dialysis tube that contains 10 mL of a 5% sucrose solution and that is permeable to water but not sucrose is placed in 100 mL of a 5% sucrose solution. What is the tonicity of the solution in the beaker? hypertonic hypotonic isotonic cannot be determined