Assume you used a hemocytometer to count your cells. Given the results below, calculate the total cell count (aka total cell #) in your cell culture sample. Cells Counted: 650 Squares Counted: 3 Dilution factor: not diluted Total cell suspension volume: 2mL

Human Anatomy & Physiology (11th Edition)
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ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
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Assume you used a hemocytometer to count your cells. Given the results below, calculate the total
cell count (aka total cell #) in your cell culture sample.
Cells Counted: 650
Squares Counted: 3
Dilution factor: not diluted
Total cell suspension volume: 2mL
Transcribed Image Text:Assume you used a hemocytometer to count your cells. Given the results below, calculate the total cell count (aka total cell #) in your cell culture sample. Cells Counted: 650 Squares Counted: 3 Dilution factor: not diluted Total cell suspension volume: 2mL
Expert Solution
Step 1: Understanding the instrument:

A hemocytometer is a specialised counting chamber used in laboratories to count and gauge the quantity of cells in liquid samples, notably blood cells.

A shallow rectangular chamber or well has been etched into the surface of a thick glass microscope slide, which makes up the device. Usually, a grid of smaller squares or rectangles divides the chamber.

Hemocytometerseses are commonly used in medical and biological laboratories for tasks such as:

  1. Cell Counting
  2. Sperm Counting
  3. Cell Viability Assays
  4. Yeast and Microbial Cell Counting
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