When analyzing your results after performing a serial dilution, you obtain an average of 40 colonies on your 1 x 106 plate. How many cells were in the original sample?

Q: You look at a slide you prepared and, despite the fact that you know you put organism onto the slide…

A: Gram-staining is a laboratory test that detects the presence of bacteria and also helps to…

Q: What would your final dilution be if you transferred 0.1 mL of a 10-6 dilution and added it to a…

A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…

Q: Meats are a poor source of folate because folate is destroyed by ______________________but you can…

A: Folate is naturalky present in wide variety of food like meat and raw vegitables .

Q: How could you utilize fluorescence quenching in the different analytical application? Please answer…

A: Fluorescence quenching is a significant method for estimating restricting partiality among ligands…

Q: You have a culture that has 5 X103 cfu/ml of bacteria in it. You perform a serial dilution by adding…

A: In microbiology and bacteriology, the technique of serial dilution is a fundamental method used to…

Q: Three grams of soil from the ground were added to 27 mL of sterile water and shaken vigorously.…

A: The initial dilution was a 1/100 dilution, followed by 4 1/10 dilutions. So, the calculate the CFU…

Q: If you were working with an unlabeled simple stained smear, would you be able to identify the…

A: Staining is used to colour biological specimen such as cell to identify them. Simple staining…

Q: You aseptically transfer 1 ml of your optional liquid culture into 99 ml of sterile water. What is…

A: The following are needed for calculating the dilution factor  1) Original volume of  solution we…

Q: If you transfer 0.1 mL of culture into a 99mL of sterile water, then add 1mL of that to an agar…

A: Making a solution thinner or less dense is the procedure of dilution. Serial dilutions (also known…

Q: Why is it important to use a sterilized loop between streaks when preparing a streak­plate?…

A: Streak plates are prepared to grow and isolate individual colonies of bacteria. Here, we will mark…

Q: What are the advantages and disadvantages of viable cell count and the turbidimetric methods?

A: In turbidimetric determination of cell growth ,after inoculation of the bacterial culture the…

Q: You are incubating cells for 3 hours. The initial number of cells present in the sample were 102.…

A: Generation time is the time interval required for cells or a population to divide. Generation time…

Q: Which of the following methods of enumerating cells is the only one to detect live cells?

A: The cell culture is the process by which cells are grown under controlled conditions, generally…

Q: Five different strains of E. coli (1, 2, 3, 4 and 5) were streaked onto a series of tester plates.…

A: Having a minimal medium decides what exactly is used by the bacteria that is under study for its…

Q: How do you know if you have a ‘countable’ plate when doing a dilution series?   Two plates…

A: The concentration of stock solution can be calculated by first diluting the stock into a suitable…

Q: To determine the number of cells in a pure culture of Klebsiella pneumoniae, you have performed a…

A: Colony-forming units per millilitre (cfu/ml) of Klebsiella pneumonia original culture can be found…

Q: you are given a mixed culture of s. aureus, E. coli and P. aeruginosa. how would you isolate each…

A: In the study of microorganisms, a mixed culture is one that contains more than one type of organism…

Q: If the field diameter is 2.4 mm and there are 16 cells across the field of view when using the 40X…

A: Given, Field diameter - 2.4 mm Cells across the field of view - 16 cells Objective Lens - 40X The…

Q: You aseptically transfer 1ml of your original liquid culture into 999 ml of sterile water. What is…

A: The dilution factor may be expressed as the ratio of the concentration of stock solution to the…

Q: The actual length of a cell is 50µm. When projected on the big screen in the class, the same cell is…

A: Microscopy is a technique that is used to visualize objects that are not visible to the naked eyes.…

Q: What was the concentration of bacteria (CFU/mL) in the original suspensions used for dilutions and…

A: The ideal colony size is found between 30 and 300. The CFU/ml is calculated by: (Colony × Dilution…

Q: You perform the Methyl-Red (right) and Voges-Proskauer test (left). Interpret the results. Which…

A: Voges-Proskauer (VP) is a test used to determine the acetoin in broth culture of bacteria. VP broth…

Q: The actual length of a cell is 50µm. When projected on the big screen in the class, the same cell is…

A: Microscopy is one of the basic processes on the basis of which the whole field of Biology rests.…

Q: A sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it.…

A: A serial dilution is a process of sequential dilution in order to decrease the density of cell…

Q: In this experiment, different concentrations of sucrose are first prepared. Then, cylinders of…

A: Water Potential: Water plays a significant role in the living systems. It is a universal solvent, it…

Q: You have performed an experiment and your aim is to obtain pure colonies. However, you observe some…

A: Bacteria are prokaryotic organisms. They are microscopic single-celled organisms that can live in a…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.