6.1 EXPECTED RESULT FOR CELL COUNT USING HAEMOCYTOMETER Table 2 Number of cell in upper Grid location number Number of cell in lower grid 20 grid 1 15 13 17 4 10 14 18 13 20 15 13 7 9 17 10 14 10 12 13 18 Total cells in upper and lower grid 120 120 TOTAL CELLS 240 Total grids 16 Please calculate a numbers of cell/ml from the information table 2.

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6.1 EXPECTED RESULT FOR CELL COUNT USING HAEMOCYTOMETER
Table 2
Number of cell in upper
grid
15
13
Grid location number
Number of cell in lower
grid
20
17
1
3
4
10
14
18
13
7
20
15
17
14
13
10
10
12
13
18
Total cells in upper
and lower grid
120
120
TOTAL CELLS
240
Total grids
16
Please calculate a numbers of cell/ml from the information table 2.
Transcribed Image Text:6.1 EXPECTED RESULT FOR CELL COUNT USING HAEMOCYTOMETER Table 2 Number of cell in upper grid 15 13 Grid location number Number of cell in lower grid 20 17 1 3 4 10 14 18 13 7 20 15 17 14 13 10 10 12 13 18 Total cells in upper and lower grid 120 120 TOTAL CELLS 240 Total grids 16 Please calculate a numbers of cell/ml from the information table 2.
Cell counting calculation
Basic hemacytometer formula
Neubauer Hemacytometer
Calculation
Number of cells counted X dilution factor
total volume of the cells counted
total volume - # primary squares x 0.1 (deptn)
Cells X dilution = cells/uL
squares X 0.1
This formula can be used with any counting
chamber as long as one knows the
dimensions for the chamber
Note: 0.1 mm depth is constant in Neubauer Hemacytometer
Size
Volume
0.0025 mm
0.04 mm
0.0625 mm
1 mm
Blue
0.25 nl
Yellow
4 nl
Green
6.25 nl
Red
100 nl.
average live cells x dilution factor
volume of a square (mL)
Cell density (cells/mL) =
i.
Example calculation
Given information bellows:
No of yeast cells in 14 grids (yellow grids) = 210
No cells in average = 210/14
= 15 cells
Dilution factor = 1
Total volume = Area x Depth
= 0.04mm? x 0.1mm (From haemocytometer)
= 0.004mm3
= 4.0 x 106 ml
Cells/ml = (15cells x 1) /4.0 x 106 ml
%3D
= 3.8 x 10° cell/ml
Transcribed Image Text:Cell counting calculation Basic hemacytometer formula Neubauer Hemacytometer Calculation Number of cells counted X dilution factor total volume of the cells counted total volume - # primary squares x 0.1 (deptn) Cells X dilution = cells/uL squares X 0.1 This formula can be used with any counting chamber as long as one knows the dimensions for the chamber Note: 0.1 mm depth is constant in Neubauer Hemacytometer Size Volume 0.0025 mm 0.04 mm 0.0625 mm 1 mm Blue 0.25 nl Yellow 4 nl Green 6.25 nl Red 100 nl. average live cells x dilution factor volume of a square (mL) Cell density (cells/mL) = i. Example calculation Given information bellows: No of yeast cells in 14 grids (yellow grids) = 210 No cells in average = 210/14 = 15 cells Dilution factor = 1 Total volume = Area x Depth = 0.04mm? x 0.1mm (From haemocytometer) = 0.004mm3 = 4.0 x 106 ml Cells/ml = (15cells x 1) /4.0 x 106 ml %3D = 3.8 x 10° cell/ml
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