If you have cells that are larger and contain more chlorophyll per cell than the cells shown in the following flow cytometric signature, where would you expect them to likely show up when they are run through the flow cytometer and why

Q: Why does counting 5 Boxes in each chamber of the hemacytometer gives you the number of cells in a ul…

A: Hemacytometer is a medical instrument used to measure the concentration of blood components in a…

Q: Use your knowledge of field diameter to calculate the diameter of a Paramecium that takes up 1/3 of…

A: This problem revolves around the calculation of the diameter of a Paramecium, a type of…

Q: You are designing a medical device to collect Carcinoembryonic Antigen (CEA, MW = 180,000 g mol^-1),…

A: Explanation: -Within the extraction chamber, the diffusivity of CEA is determined to be 6.1 x 10-6…

Q: I am not sure how to go about illustrating and answering this question: Use a diagram to illustrate…

A: The serial dilution is a very important process that is routinely used in microbiology and cell…

Q: Using my chart information can you help me indicate where the four solutes were present before and…

A: Diffusion through a selectively permeable membrane refers to the movement of molecules from higher…

Q: In making the Feta Cheese, a 10% Calcium Chloride solution was added. Explain why you think that…

A: During the preparation of Feta cheese, different ingredients are used. 10% Calcium chloride solution…

Q: Tube 1 Tube 2 Tube 3 Germinating Nongerminating Soybeans Glass Beads Soybeans Time (min) Fluid Level…

A: Given  For germinating seeds (ml) Level of oxygen at 0 minut = 8.2  Level of oxygen at 10 minut =…

Q: You want to add cells at a density of 3 x 104 cells / cm2 to a 12-well plate where each well has an…

A: Cell density:It is very tightly regulated and varies very little among given cell type. Changes in…

Q: The dot plot above shows cells that have been characterised by forward and side scatter. Forward…

A: Fluorescent dyes can be used to detect and monitor pollutants in the environment. Fluorescent dyes,…

Q: Please count the cells in grids 1, 3, 7, and 9. Calculate the cell density (a) and cell viability…

A: The hemocytometer was first discovered by Louis Charles Malassez to count blood cells, hence, the…

Q: Based on the following information acquired using a hemocytometer, calculate: i) cell concentration*…

A: In a hemocytometer, both the stained (viable) and unstained (non-viable) cells are counted in…

Q: Create a schematic diagram of the experimental procedure down below

A: Chlorophyll is the pigment contained in special membrane-enclosed organelles called "chloroplasts"…

Q: Explain the working principle of a flow cytometer. List its applications.

A: Flow cytometer is an analytical technique which is used for analysis of single particle or cell.…

Q: Please answer fast 1:10 dilution is made of a solution. DRAW how you would make a further 1:10,000…

A: Make a 1:10 dilution of a 1M NaCl solution, so mix the one "part" of the 1M solution with nine…

Q: What is a threshold setting (also called a trigger) used for when acquiring flow cytometric data?…

A: A threshold setting is used to determine when data acquisition should start and stop.

Q: You have cultured some cells for a flow cytometry experiment. The cell concentration has been…

A: A method for detecting and analysing the physical and chemical characteristics of cells and other…

Q: Suppose you are handling a P 200-1000 µl micropipette and while adjusting the volume you go over the…

A: Micropipette is an instrument used to measure volumes in microliters in the laboratory. The art of…

Q: You are given a cell stock A. You make a 1:100 dilution and load 10pL diluted sample to a…

A: A hemocytometer is an important tool in the laboratory for RBC and WBC count. An original cell…

Q: Suppose you have a suspension of HeLa cells with a cell count of 85,000 cellsper ml, and you want to…

A: HeLa cells were discovered in 1950s and is named after the women from whom these cells are taken…

Q: You are designing a medical device to collect Carcinoembryonic Antigen (CEA, MW = 180,000 g mol^-1),…

A: Explanation: The mass transfer coefficient is a measure of the rate of transfer of a substance from…

Q: Questions 8 - What tonicity would best describe Beaker E and why? Questions 9 - What tonicity would…

A: Homeostasis means maintaining balance. Beaker A fill with 1000 ml water, that means beaker A doesn't…

Q: lue-green algae be

A: Cyanobacteria are a group of photosynthetic bacteria that were previously referred to as "blue-green…

Q: Draw cells in 4 large squares of the hemocytometer showing: 48 total viable cells (hollow dots) 9…

A: Hemocytometer is a counting- device used for counting blood cells. It is a glass slide with a cover…

Q: 29. B Required The diagram below represents what occurred when an onion (plant) cell and a red blood…

A: Introduction :  Osmosis is a passive process that takes place with no energy required. When the…

Q: Using the date in the photo of the two syringes below, answer these six questions: QUESTIONS 1.…

A: The given data and the graph represent the relationship with the photosynthesis process. The…

Q: The birt rate, b, is defined by what equations:  b=r+d b= r-d b= Bt/Nt b= r/d b= Bt-Nt

A:

Q: Explain the answers in detail. a. Discuss briefly X-ray absorption and filtration.

A: We are answering First question. For rest of questions pls repost.     Electromagnetic waves are a…

Q: n this part of the assignment, assume you have carried out the microscopic examination of the…

A: Depending on the amount of solute and solvent present, a solution is classified into three…

Q: Standard curves You are setting up a standard curve for lysozyme. You have a stock vial containing 2…

A: Proteins are chains of amino acids bonded via amide bonds. Lysozyme is an antimicrobial enzyme that…

Q: what is the concentration of cells per 1 mL of solution?

A: Hemocytometer is also known as the cell counting chamber, is primarily used for counting cells…

Q: There are three main subsystems to a flow cytometer. Describe the primary function of each one in a…

A: Flow cytometry is basically an bioinstrumental technique which is implicated to detect and quantify…

Q: Choose a range of OD 600 from the data set that can assure a good correlation of viable cell count…

A: Good and viable cells are the cells that are obtained from the log phase of the bacterial cycle. For…

Q: You are counting WBC using a hemocytometer, consider that you use only 2 squares (2 W) in your…

A: Hemocytometer -- A hemocytometer , manual way for counting White Cell Counting . It can be performed…

Q: Please explain briefly the role of hemocytometer in this experiment and give some general…

A: Introduction The Hemocytometer Resembles A Standard Glass Slide From A Distance, But It Is Far More…

Q: Discuss what is meant by the Oxygen effect, the oxygen enhancement ratio and some possible…

A: Since you have posted a question with multiple sub-parts, we will solve the first three sub-parts…

Q: You put some Saccharomyces cereviciae stock concentration in a hemocytometer and count the 1/25mm^2…

A: 1.Calculate the average count:(12 + 23 + 34 + 16 + 14 + 2) / 6 = 101 / 6 ≈ 16.83Round this to the…

Q: Look at the slide . Count the number of cells in each quadrant. How many cells are in the sample,…

A: Hemocytometer is a device that was designed by the French anatomist Louis-Charles Malassez to count…

Q: Questions 5 - What molecule moved into or out of the dialysis tubes in this experiment? Questions 6…

A: Homeostasis is the steady state internal, physical, and chemical conditions maintained by living…

Q: 3. (a) What are the different methods of cell lysis? Discuss in detail.

A: Cell disruption is the process of rupturing (or lysing) cells in order to obtain the intracellular…

Q: What are two properties or characteristics of a cell or particle that can be measured by a flow…

A: Definition: - Flow cytometer is a technology that makes use of multi parametric analysis of cells in…

Q: Plate A = filter paper dipped into 5% solution of unknown Dye A Plate B = filter paper dipped into…

A: Transport is defined as the movement of molecules from one region to another region.

Q: Two patients have the same Oxygen Saturation value, but the second patient (2) has a reduced red…

A: as per our company guidelines we are supposed to answer only first 1 question. Kindly repost other…

Q: Imagine that you are viewing a slide with a heat-fixed smear from an sputum sample that has been…

A: Hi! Thank you for the question as per the honour code, we’ll answer the first question since the…

Osmosis

In simple words, osmosis is the slow, gradual movement of a liquid through a membrane. But there are nuances to this process. For instance, there has to be an imbalance in the distribution of solute in the liquid on either side of the membrane, which has to be semipermeable. The liquid in the region with a lower solute concentration moves to the region with a higher concentration, thereby displacing the solution in the process. The objective is to equalize the concentration of molecules on both sides of the membrane; the displacement of liquid can be viewed as a byproduct of this equalization. Since there is no energy required to carry osmosis, it is considered a passive process.