Answer these two You are given cell stock A. After you load and count, the average number of cells in one counting square is 35. Now youcan use this number to calculate the concentration of this cell stock. The concentration isSelect one:a. 3.5x105 cells/mLb.3500 ce 'mLC.350 cells/mLO d. 3.5x104ª cells/mLFor cell stock A, you made a 1:100 dilution and figured out the concentration is 3.3x105 cells/mL. The concentration ofthe original stock (before dilution) isSelect one:a. 3.3x103cell/mLb. 3.3x105cells/mLc. 3.3x10 cells/mLd. 3.3x10 cells/mLIInder a light microscone, the number of cells in each counting square is counted and listed. What is the average

To calculate cell concentration, take the average number of cells in four sets of 16 squares and…

You are given cell stock A. After you load and count, the average number of cells in one counting square is 35. Now you can use this number to calculate the concentration of this cell stock. The concentration is

You are given cell stock A. After you load and count, the average number of cells in one counting square is 35. Now you can use this number to calculate the concentration of this cell stock. The concentration is

Comprehensive Medical Assisting: Administrative and Clinical Competencies (MindTap Course List)

6th Edition

ISBN:9781305964792

Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Chapter30: Assisting With Minor Surgery

Section: Chapter Questions

Problem 2CR

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You are given a cell stock A. You make a 1:100 dilution and load 10µL diluted sample to a hemocytometer. After counting all 5 "one millimeter squares" you get an average of 60 cells. Then you decide to do a 1:1000 dilution to verify your counting. How many cells do you expect to get in average with 1:1000 dilution? Select one: a. 6 b. 12 с. 30 O d. 20 To do a 1:100 serial dilution. This is what you will do: Select one: a. You take 1uL of original cell stock and add into 99 uL of 1×PBS to make a 1:100 dilution b. You take 90uL of cell stock and add that into a tube containing 10uL of 1x PBS. c. You take 99uL of cell stock and add that into a tube containing 10uL of 1×PBS. d. You take 10uL of original cell stock and add into 90 uL of 1x PBS to make a 1:10 dilution. You thoroughly mix the 1:10 dilution tube. Then you take 10uL out from 1:10 tube and add it into a new tube containing 90uL of 1×PBS.
Clear my choice You have a yeast cell stock in a flask. The total volume is 10mL. After counting and calculation, you figure out the average number of cells in the counting square is 15. The total number of cells in the flask is Select one: a. 150 cells b. 1.5x10 cells/mL O c. 1.5x105cells/mL d. 1.5x10 cells Clear my choice In animal cells, is used to distinguish between living and dead cells
Which of the following is false about counting viable cells? A. CFU(Colony forming unit) are counted instead of individuals cells B. Only 30-300 CFUs are statistically valid C. Colonies growing on the plate represent a fraction of the live cells in the original sample D. You can determine the number of live cells in a broth culture E. It is necessary to multiply the by CFUs to determine the number of viable cells in the original sample
A 5mL sample diluted 1:1 with trypan blue is counted using a hemocytometer. A total of 325 viable cells and 52 dead cells were counted in the four corner square and the big central square of the meter. What is the total number of viable cells in the original sample? * A. 8.0 x 10^6 B. 3.3 x 10^7 C. 4.1 x 10^7 D. 6.5 x 10^6
You have an original cell density of 4.3x10 8CF/mL. What is this number in its non-scientific notation or "regular" format?. 43,000,000CF/mL. 0.000000043CfU/mL. 430,000,000CFU/mL. 0.00000043CFU/mL. 4.3CFU/mL
What is the percentage of viable cells shown in the image? 10 O
I asked earlier a question, and I got this answer. Now, my question is: how do you get the 2.5cm= 100um? I have taken a picture of the paramecium in question, with the scale bar (100um), and a ruler showing it’s measurement is around 2.5cm. Is that how we got the 2.5?
Please count the cells in grids 1, 3, 7, and 9. Calculate the cell density (a) and cell viability (b) for this sample. (assume we started with 100 microliters of cells and added 100 microliters of trypan viability blue). Hemacytometer Grid 123 5 6 789 4 Enlarged view O O O 8
You counted 4, 6, 12, 3 cells in each of the 4.outed squares of a hemacytomeyer. What are the cells per milliliter in that culture? If you resuspended your cell pellet 2.5 mL, what is the total cell count? How many uL do you need to add to a new culture if you want 4250 cells?
You want to subculture your cells from T25 flask to 96-well plates. You first collected your cells in a tube with 5ml of culture medium. Then carried out a trypan blue assay and counted your cells with a hematocytometer as shown in Figure below. Answer the questions according to the results: a. Calculate the concentration of the stock including the dead and living cells. Dont forget to show the units! b. Calculate the percentage (%) of the living cells in the stock. c. You want to seed 6000 living cells into each well of 96 well plates, then calculate the volume you should take from the stock for each well. 輯 1 mm I IRE
Can you please answer question 5. According to your answers provide at question 2 there should be a certain number of bands visible on your gel. Suppo. you did this practical and you observed less bands on your gel, give possit I explanations for this.
MULTIPLE CHOICE: Select the BEST ANSWER for each item by ENCIRCLING THE LETTER corresponding to the best choice. 24. The following are requirements during culture maintenance, EXCEPT: Maintain sterility C. Maintain optimal temperature Maintain optimal humidity A. В. Arrest cell division D. 25. Aging of slides containing harvested cells enhances chromosome banding. Which of the following is NOT an aging technique? Overnight at 60°C C. One hour at 90°C D. Brief exposure to ultraviolet light Exposure to barium hydroxide A. В. 26. Chromosome analysis of mature lymphocytes from a female patient revealed the following: Total number of chromosomes counted = 45; whole chromosome probe for sex chromosomes highlighted or "painted" only one X chromosome. Which of the following statements is consistent with the FISH findings of this patient? А. Patient has autosomal monosomy. Patient has Klinefelter syndrome. C. Patient has Turner syndrome. Patient has Edwards syndrome. В. D. What does…