Answer these two You are given cell stock A. After you load and count, the average number of cells in one counting square is 35. Now youcan use this number to calculate the concentration of this cell stock. The concentration isSelect one:a. 3.5x105 cells/mLb.3500 ce 'mLC.350 cells/mLO d. 3.5x104ª cells/mLFor cell stock A, you made a 1:100 dilution and figured out the concentration is 3.3x105 cells/mL. The concentration ofthe original stock (before dilution) isSelect one:a. 3.3x103cell/mLb. 3.3x105cells/mLc. 3.3x10 cells/mLd. 3.3x10 cells/mLIInder a light microscone, the number of cells in each counting square is counted and listed. What is the average

To calculate cell concentration, take the average number of cells in four sets of 16 squares and…

You are given cell stock A. After you load and count, the average number of cells in one counting square is 35. Now you can use this number to calculate the concentration of this cell stock. The concentration is

You are given cell stock A. After you load and count, the average number of cells in one counting square is 35. Now you can use this number to calculate the concentration of this cell stock. The concentration is

Comprehensive Medical Assisting: Administrative and Clinical Competencies (MindTap Course List)

6th Edition

ISBN:9781305964792

Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Chapter30: Assisting With Minor Surgery

Section: Chapter Questions

Problem 2CR

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You are given a cell stock A. You make a 1:100 dilution and load 10µL diluted sample to a hemocytometer. After counting all 5 "one millimeter squares" you get an average of 60 cells. Then you decide to do a 1:1000 dilution to verify your counting. How many cells do you expect to get in average with 1:1000 dilution? Select one: a. 6 b. 12 с. 30 O d. 20 To do a 1:100 serial dilution. This is what you will do: Select one: a. You take 1uL of original cell stock and add into 99 uL of 1×PBS to make a 1:100 dilution b. You take 90uL of cell stock and add that into a tube containing 10uL of 1x PBS. c. You take 99uL of cell stock and add that into a tube containing 10uL of 1×PBS. d. You take 10uL of original cell stock and add into 90 uL of 1x PBS to make a 1:10 dilution. You thoroughly mix the 1:10 dilution tube. Then you take 10uL out from 1:10 tube and add it into a new tube containing 90uL of 1×PBS.
Clear my choice You have a yeast cell stock in a flask. The total volume is 10mL. After counting and calculation, you figure out the average number of cells in the counting square is 15. The total number of cells in the flask is Select one: a. 150 cells b. 1.5x10 cells/mL O c. 1.5x105cells/mL d. 1.5x10 cells Clear my choice In animal cells, is used to distinguish between living and dead cells
You collected 1000 mL of river water from the stream running through campus. You prepared dilutionsof the water as follows:Tube A. 0.5 mL of river water + 99.5 mL of sterile waterTube B. 1 mL from Tube A + 9 mL of sterile waterTube C. 1 mL from Tube B + 9 mL of sterile waterTube D. 1 mL from Tube C + 9 mL of sterile waterYou then plated 0.1 mL of each dilution onto each of three MacConkey agar plates. (MacConkey agar is type of selective,differential medium. It inhibits the growth of Gram-positive microorganisms and non-enteric Gram-negative bacteria.Among the bacteria not inhibited by the medium, lactose-fermenting bacteria form pink colonies and bacteria that do notferment lactose form colorless colonies.) After incubation, the colonies were too numerous to count on the platesprepared from dilutions A, B, and C. For the plates prepared from dilution D you counted the following numbers ofcolonies:Plate 1: 155 pink colonies and 50 colorless coloniesPlate 2: 165 pink colonies and 55…
You collected 1000 mL of river water from the streamrunning through campus. You prepared dilutions of the water as follows:Tube A. 0.5 mL of river water + 99.5 mL of sterile waterTube B. 1 mL from Tube A + 9 mL of sterile waterTube C. 1 mL from Tube B + 9 mL of sterile waterTube D. 1 mL from Tube C + 9 mL of sterile waterYou then plated 0.1 mL of each dilution onto each of three MacConkey agarplates. (MacConkey agar is type of selective, differential medium. It inhibitsthe growth of Gram-positive microorganisms and non-enteric Gram-negative bacteria. Among the bacteria not inhibited by the medium,lactose-fermenting bacteria form pink colonies and bacteria that do notferment lactose form colorless colonies.) After incubation, the colonieswere too numerous to count on the plates prepared from dilutions A, B, andC. For the plates prepared from dilution D you counted the followingnumbers of colonies:Plate 1: 157 pink colonies and 62 colorless coloniesPlate 2: 167 pink colonies and 67…
When doing a routine differential count for a CBC how many cells are counted? a) 50 b 75 c 100 d 125 e 150
You have an original cell density of 4.3x10 8CF/mL. What is this number in its non-scientific notation or "regular" format?. 43,000,000CF/mL. 0.000000043CfU/mL. 430,000,000CFU/mL. 0.00000043CFU/mL. 4.3CFU/mL
What is the percentage of viable cells shown in the image? 10 O
I asked earlier a question, and I got this answer. Now, my question is: how do you get the 2.5cm= 100um? I have taken a picture of the paramecium in question, with the scale bar (100um), and a ruler showing it’s measurement is around 2.5cm. Is that how we got the 2.5?
Please count the cells in grids 1, 3, 7, and 9. Calculate the cell density (a) and cell viability (b) for this sample. (assume we started with 100 microliters of cells and added 100 microliters of trypan viability blue). Hemacytometer Grid 123 5 6 789 4 Enlarged view O O O 8
You counted 4, 6, 12, 3 cells in each of the 4.outed squares of a hemacytomeyer. What are the cells per milliliter in that culture? If you resuspended your cell pellet 2.5 mL, what is the total cell count? How many uL do you need to add to a new culture if you want 4250 cells?
a. 40x b.10x C. None D. 4x
Assume that you are starting with a sample containing 6 billion active cells. Create a dilution plan to reduce this count to a range of 30-300 colonies on a plate. The powdered sample weighs 500 mg (0.5 g). You can use up to 4 plates – you should plate different dilutions and/or volumes of solution. Start your first dilution by adding 10 mL diluent to 0.5 g powder, which is a 1/20 dilution. Hint: Rearranging the “cfu/ml” formula from our dilutions lab can help you solve this. Choose a target cfu count, and your volume plated should be under 0.5 ml.