You are given a cell stock A. You make a 1:100 dilution and load 10µL diluted sample to a hemocytometer. After counting all 5 "one millimeter squares" you get an average of 60 cells. Then you decide to do a 1:1000 dilution to verify your counting. How many cells do you expect to get in average with 1:1000 dilution? Select one: a. b. 12 O c. 30 d. 20 To do a 1:100 serial dilution. This is what you will do: Select one: You take 1uL of original cell stock and add into 99 uL of 1XPBS to make a 1:100 dilution a. b. You take 90uL of cell stock and add that into a tube containing 10uL of 1x PBS. C. You take 99uL of cell stock and add that into a tube containing 10uL of 1XPBS. d. You take 10uL of original cell stock and add into 90 uL of 1x PBS to make a 1:10 dilution. You thoroughly mix the 1:10 dilution tube. Then you take 10uL out from 1:10 tube and add it into a new tube containing 90uL of 1×PBS.
You are given a cell stock A. You make a 1:100 dilution and load 10µL diluted sample to a hemocytometer. After counting all 5 "one millimeter squares" you get an average of 60 cells. Then you decide to do a 1:1000 dilution to verify your counting. How many cells do you expect to get in average with 1:1000 dilution? Select one: a. b. 12 O c. 30 d. 20 To do a 1:100 serial dilution. This is what you will do: Select one: You take 1uL of original cell stock and add into 99 uL of 1XPBS to make a 1:100 dilution a. b. You take 90uL of cell stock and add that into a tube containing 10uL of 1x PBS. C. You take 99uL of cell stock and add that into a tube containing 10uL of 1XPBS. d. You take 10uL of original cell stock and add into 90 uL of 1x PBS to make a 1:10 dilution. You thoroughly mix the 1:10 dilution tube. Then you take 10uL out from 1:10 tube and add it into a new tube containing 90uL of 1×PBS.
Comprehensive Medical Assisting: Administrative and Clinical Competencies (MindTap Course List)
6th Edition
ISBN:9781305964792
Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa
Publisher:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa
Chapter30: Assisting With Minor Surgery
Section: Chapter Questions
Problem 2CR
Related questions
Question
![You are given a cell stock A. You make a 1:100 dilution and load 10µL diluted sample to a hemocytometer. After counting
all 5 "one millimeter squares" you get an average of 60 cells. Then you decide to do a 1:1000 dilution to verify your
counting. How many cells do you expect to get in average with 1:1000 dilution?
Select one:
a. 6
b. 12
с. 30
O d. 20
To do a 1:100 serial dilution. This is what you will do:
Select one:
a.
You take 1uL of original cell stock and add into 99 uL of 1×PBS to make a 1:100 dilution
b. You take 90uL of cell stock and add that into a tube containing 10uL of 1x PBS.
c. You take 99uL of cell stock and add that into a tube containing 10uL of 1×PBS.
d. You take 10uL of original cell stock and add into 90 uL of 1x PBS to make a 1:10 dilution. You thoroughly mix the
1:10 dilution tube. Then you take 10uL out from 1:10 tube and add it into a new tube containing 90uL of 1×PBS.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fe2bbef39-13a9-4a3a-ae0f-bc83a28a95a1%2Fdfe5bfa2-dd8b-42b3-86c5-e190913083df%2F5y60bjq_processed.jpeg&w=3840&q=75)
Transcribed Image Text:You are given a cell stock A. You make a 1:100 dilution and load 10µL diluted sample to a hemocytometer. After counting
all 5 "one millimeter squares" you get an average of 60 cells. Then you decide to do a 1:1000 dilution to verify your
counting. How many cells do you expect to get in average with 1:1000 dilution?
Select one:
a. 6
b. 12
с. 30
O d. 20
To do a 1:100 serial dilution. This is what you will do:
Select one:
a.
You take 1uL of original cell stock and add into 99 uL of 1×PBS to make a 1:100 dilution
b. You take 90uL of cell stock and add that into a tube containing 10uL of 1x PBS.
c. You take 99uL of cell stock and add that into a tube containing 10uL of 1×PBS.
d. You take 10uL of original cell stock and add into 90 uL of 1x PBS to make a 1:10 dilution. You thoroughly mix the
1:10 dilution tube. Then you take 10uL out from 1:10 tube and add it into a new tube containing 90uL of 1×PBS.
Expert Solution
![](/static/compass_v2/shared-icons/check-mark.png)
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
![Blurred answer](/static/compass_v2/solution-images/blurred-answer.jpg)
Recommended textbooks for you
![Comprehensive Medical Assisting: Administrative a…](https://www.bartleby.com/isbn_cover_images/9781305964792/9781305964792_smallCoverImage.gif)
Comprehensive Medical Assisting: Administrative a…
Nursing
ISBN:
9781305964792
Author:
Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa
Publisher:
Cengage Learning
![Comprehensive Medical Assisting: Administrative a…](https://www.bartleby.com/isbn_cover_images/9781305964792/9781305964792_smallCoverImage.gif)
Comprehensive Medical Assisting: Administrative a…
Nursing
ISBN:
9781305964792
Author:
Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa
Publisher:
Cengage Learning