tood time Ime Iml Iml ml Tml 99 99 99 9. 0.1 ml 0/-1 ml T5A plates TMTC 219 2나 colonies bacteria in oug food

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Answer the worksheet 

Food
imd
Lml
Ime
Iml
Ime
Tml
Tml
99
99
99
9
0/1 ml
0.1 ml
0/.1 ml
TSA plates
TMTC
219
24
%#3
colonies
bacteia
in
oug food
Transcribed Image Text:Food imd Lml Ime Iml Ime Tml Tml 99 99 99 9 0/1 ml 0.1 ml 0/.1 ml TSA plates TMTC 219 24 %#3 colonies bacteia in oug food
Expert Solution
Step 1

Pour plate method:

  • The technique is performed to count the number of microbial loads in a sample.
  • In this procedure, 1 mL of inoculum is taken using a sterile pipette and placed at the center of the Petri plate. 
  • Approximately 15 mL of cooled agar is poured into the plate, and it is mixed with inoculum.
  • The plate is incubated for 24 to 48 hours, and the number of microorganisms present in the sample is calculated.  
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