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tood time Ime Iml Iml ml Tml 99 99 99 9. 0.1 ml 0/-1 ml T5A plates TMTC 219 2나 colonies bacteria in oug food
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Expert Solution
Step 1
Pour plate method:
- The technique is performed to count the number of microbial loads in a sample.
- In this procedure, 1 mL of inoculum is taken using a sterile pipette and placed at the center of the Petri plate.
- Approximately 15 mL of cooled agar is poured into the plate, and it is mixed with inoculum.
- The plate is incubated for 24 to 48 hours, and the number of microorganisms present in the sample is calculated.
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