TRUE or FALSE only! 1. Pure cultures contain only a single species and no other organisms. 2. Pour plate method is done by pipetting liquified warm agar into a petri dish containing an inoculum. 3. Flaming the test tube’s mouth is necessary so that the moisture that accumulated will evaporate. 4. After Gram staining, the Gram positive bacteria will appear blue while the Gram negative bacteria will appear red.

TRUE or FALSE only! 1. Pure cultures contain only a single species and no other organisms. 2. Pour plate method is done by pipetting liquified warm agar into a petri dish containing an inoculum. 3. Flaming the test tube’s mouth is necessary so that the moisture that accumulated will evaporate. 4. After Gram staining, the Gram positive bacteria will appear blue while the Gram negative bacteria will appear red.

An Illustrated Guide To Vet Med Term

4th Edition

ISBN:9781305465763

Author:ROMICH

Publisher:ROMICH

Chapter11: The Great Communicator

Section: Chapter Questions

Problem 14MC

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3. A bacterial culture was diluted and results from duplicate plates were obtained as indicated below. What was the number of colony forming units/mL of the original culture? Dilution used for plating 10-2 10-3 104 10-5 10⁰ 10-7 10-8 4. 5. Amount plated 0.1 mL 0.1 mL 0.1 mL 0.1 mL 0.1 mL 0.1 mL 0.1 mL Colony counts after incubation (Results from duplicate plates) Too many to count Too many to count 341 413 99 175 27 : 29 7:2 0:0 Ten grams of hamburger were added to 90 mL of sterile buffer. This was mixed well in a blender. One-tenth of a mL of this slurry was added to 9.9 mL of sterile buffer. After thorough mixing, this suspension was further diluted by successive 1/100 and 1/10 dilutions. One-tenth of a mL of this final solution was plated onto Plate Count agar. After incubation 145 colonies were present. How many colony-forming units were present in the total 10 gram sample of hamburger? Devise a serial dilution scheme to prepare a 10-5 dilution on a plate using the least number of…
Tell me 3 things about each one? are they selective , differential , positive , negative results etc. gram stain isolation streak ftm tubes
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1. You have a culture of yeast that is at a density of 8x107 cells/mL 2. A lab technician took a fecal sample and carried out the following dilutions: 1. Transferred 0.1 mL from the fecal sample 0.1 ml I ml 0.01 ml to 9.9 mL saline in tube A 2. Transferred 1 mL from A to 9 mL saline in tube B 3. Transferred 0.01 mL from B to 9.99 mL 0.1 ml A C saline in tube C 4. Transferred 0.1 mL from tube C to an agar plate 5. Counted 65 colonies on plate D 9.9 ml 9 ml 9.99 ml saline saline saline D 5. What is the concentration of cells in tube C? 6. What is the concentration of cells in the fecal sample? 1
1. You have a culture of yeast that is at a density of 8x107 cells/mL 2. A lab technician took a fecal sample and carried out the following dilutions: 1. Transferred0.1 mL from the fecal sample 0.1 ml 1 ml 0.01 ml to 9.9 mL saline in tube A 2. Transferred 1 mL from A to 9 mL saline in tube B 3. Transferred 0.01 mL from B to 9.99 mL saline in tube C 4. Transferred 0.1 mL from tube C to an agar plate 5. Counted 65 colonies on plate D 0.1 ml B 9 ml saline 9.9 ml 9.99 ml D saline saline Questions: 1. What is the serial dilution in A? 2. What is the serial dilution in B? 3. What is the serial dilution in C? 4. What is the total dilution in C? 5. What is the concentration of cells in tube C? 6. What is the concentration of cells in the fecal sample? 1
Volume of culture Start transferred for ** time serial dilution A B Volume of sterile 900 µL 900 µL 900 µL 900 µL water added to each tube prior to the transfer of bacteria Bacterial 9.9 mL culture broth at °C Volume of the diluted sample plated 20 µL 20 µL 20 µL 20 µL Plate A Plate B Plate C Plate D **Repeat this transfer at various time points into a new set of tubes
3. You were instructed to add 2.75 mL out of 5.0 mL of an undiluted sample to 125.25 mL of sterile diluent. Instead, you add all 5.0 mL to the 125.25 mL, What was the intended dilution and what was the actual dilution? ited States) E Focus OCT 30 W MacBook P DII DD F7 F8 F9 F10 F4 F5 F6 F3 2$ 4 081 R F G H. K < CO
Why is it important to limit the quantity of cells used to prepare a smear? Mark all that apply: 1. So that cells are not clumped and don't entrap stain creating erroneous results 2. So that the cells are spread out enough that cell morphology can be discerned 3. So that there are small groups of cells clumped together to make them visible 4. So that no contaminants are introduced onto the slide by being entrapped in clumps 5. So that the cells are spread out enough that the arrangement can be observed
Tell me three things about how cultures are handled in a clinical laboratory.
Answer TRUE OR FALSE only. 1. All types or kinds of media powder has a standard ratio of 23:1000. Meaning that 1000 ml of water is needed to dissolve 23 grams of powder medium. 2. Simple stains use one dye and cannot differentiate various types of bacteria while differential stains use several dyes in order to differentiate between different kinds of bacteria. 3. A certain student prepared his fixed sample for examination by pouring Nigrosin which is an acidic stain. He then observed that under the microscope the background was stained but the bacterial cells were untouched. This student therefore adapted the acid-fast staining procedure. 4. In doing serial dilutions, the original sample must be shaken at least 25 times to obtain a uniform distribution of organisms. 5. Label all reagents with its name, concentration and date of its preparation except for water.
TRUE OR FALSE 1. The antheridium of a moss could be seen as a whole mount at 100x magnification. 2. The fine adjustment knob sharpens the image in all objectives. 3. While disinfecting the isolation room with UV light, stay inside so that your hands and clothes will also be sterilized.