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Series Number [10 mg/mL] Lysozyme, mL Buffer, mL Total Vol, mL Dilution Factor [Final mg/mL] Lysozyme S1 0.5 10 S2 0.4 10 S3 0.3 10 S4 0.2 10 S5 0.1 10 Sample Prep 1. From your 1M buffer stock, prepare a 0.1 MK Phos 7.2 buffer to a final volume of 100 mL. 2. Label your conical tubes appropriately in advance ex) S1.1, S1.2, S1.3 for each triplicate in series. You should have 15 tubes in total. 3. Make up the 5 standards in triplicate (three separate dilutions of each concentration) in the appropriate concentration range, 0.1 -0.5 mg/mL as per the pre-lab assignment (3 solutions at 0.1 mg/mL, 3 solutions at 0.2 mg/mL, etc) 4. Invert each 10 mL sample in a 15 mL (or 50 mL if 15 mL unavailable) conical tube gently to mix. Let samples incubate for ~10 minutes at room temperature after making and before data acquisition.

Series Number [10 mg/mL] Lysozyme, mL Buffer, mL Total Vol, mL Dilution Factor [Final mg/mL] Lysozyme S1 0.5 10 S2 0.4 10 S3 0.3 10 S4 0.2 10 S5 0.1 10 Sample Prep 1. From your 1M buffer stock, prepare a 0.1 MK Phos 7.2 buffer to a final volume of 100 mL. 2. Label your conical tubes appropriately in advance ex) S1.1, S1.2, S1.3 for each triplicate in series. You should have 15 tubes in total. 3. Make up the 5 standards in triplicate (three separate dilutions of each concentration) in the appropriate concentration range, 0.1 -0.5 mg/mL as per the pre-lab assignment (3 solutions at 0.1 mg/mL, 3 solutions at 0.2 mg/mL, etc) 4. Invert each 10 mL sample in a 15 mL (or 50 mL if 15 mL unavailable) conical tube gently to mix. Let samples incubate for ~10 minutes at room temperature after making and before data acquisition.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Series Number [10 mg/mL] Lysozyme, mL Buffer, mL Total Vol, mL Dilution Factor [Final mg/mL] Lysozyme S1 0.5 10 S2 0.4 10 S3 0.3 10 S4 0.2 10 S5 0.1 10
Transcribed Image Text:Series Number [10 mg/mL] Lysozyme, mL Buffer, mL Total Vol, mL Dilution Factor [Final mg/mL] Lysozyme S1 0.5 10 S2 0.4 10 S3 0.3 10 S4 0.2 10 S5 0.1 10
Sample Prep 1. From your 1M buffer stock, prepare a 0.1 MK Phos 7.2 buffer to a final volume of 100 mL. 2. Label your conical tubes appropriately in advance ex) S1.1, S1.2, S1.3 for each triplicate in series. You should have 15 tubes in total. 3. Make up the 5 standards in triplicate (three separate dilutions of each concentration) in the appropriate concentration range, 0.1 -0.5 mg/mL as per the pre-lab assignment (3 solutions at 0.1 mg/mL, 3 solutions at 0.2 mg/mL, etc) 4. Invert each 10 mL sample in a 15 mL (or 50 mL if 15 mL unavailable) conical tube gently to mix. Let samples incubate for ~10 minutes at room temperature after making and before data acquisition.
Transcribed Image Text:Sample Prep 1. From your 1M buffer stock, prepare a 0.1 MK Phos 7.2 buffer to a final volume of 100 mL. 2. Label your conical tubes appropriately in advance ex) S1.1, S1.2, S1.3 for each triplicate in series. You should have 15 tubes in total. 3. Make up the 5 standards in triplicate (three separate dilutions of each concentration) in the appropriate concentration range, 0.1 -0.5 mg/mL as per the pre-lab assignment (3 solutions at 0.1 mg/mL, 3 solutions at 0.2 mg/mL, etc) 4. Invert each 10 mL sample in a 15 mL (or 50 mL if 15 mL unavailable) conical tube gently to mix. Let samples incubate for ~10 minutes at room temperature after making and before data acquisition.
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