Suppose you were instructed to repeat this experiment, again using equine albumin antiserum in two tubes. You layer equine serum (containing equine albumin) over the antiserum in one tube and pig serum (containing pig albumin) over the antiserum in the other tube. After incubation, you see precipitous rings in both tubes. Explain these results. Is this due to poor specificity or sensitivity of the test?

Suppose you were instructed to repeat this experiment, again using equine albumin antiserum in two tubes. You layer equine serum (containing equine albumin) over the antiserum in one tube and pig serum (containing pig albumin) over the antiserum in the other tube. After incubation, you see precipitous rings in both tubes. Explain these results. Is this due to poor specificity or sensitivity of the test?

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter4: Basic Immunology And Immunohematology

Section4.3: Tests For Rheumatoid Factors

Problem 2.1CS

See similar textbooks

Similar questions

What is the purpose of Biuret’s test? In biuret’s test, does the color in each test tube containing the sample vary? Why or why not?
a) did the positive and negative controls work correctly in this ELISA? Explain. B) in the sample wells( wells3-7) some are darker blue than others. Explain what happened here and how you'd interpret the results?
In this experiment, different concentrations of sucrose are first prepared. Then, cylinders of potato (Solanum tuberosum) tuber are made using a cork borer, which are then trimmed to remove the skin and be of the same length. The cylinders are blot dried and weighed prior to being placed in each of the test tubes containing the sucrose solution. After 20 minutes, the cylinders were removed from the solution, blot dried, then weighed again. Study the results of the experiment shown in Table 1. Table 1. Changes in the weights of potato tuber tissue immersed in various concentrations of sucrose for 20 minutes Concentration (M) 0.0 0.2 0.4 0.6 0.8 1.0 Initial weight 3.76 3.15 3.29 3.82 3.40 3.52 Final weight 4.44 3.40 3.09 3.32 2.71 2.57 Change in weight (g) 0.68 0.25 -0.20 -0.50 -0.69 -0.95 % Change in weight 18.09 7.94 -6.08 -13.09 -20.29 -26.99 Calculate the water potential of the potato tuber cells. Express the answer in both bars and megapascal
You are tasked with measuring the quantity of prostate specific antigen (PSA) in a urine sample using an ELISA strategy. Describe and schematically depict how you would perform the test given the following materials: anti-PSA, biotinylated anti-PSA, streptavidin-HRP, HRP substrate kit (HRP = horse radish peroxidase).
In Beadle and Tatum experiments the immediate effect of the X-ray treatment was
With the Datex-Ohmeda Aestiva/5 what are some of the service tools required for testing?
Which tube will serve as the control in the “Normal Serum Bactericidins” portion of this lab activity? Tube A (Unheated normal serum) Tube B (Normal serum heated at 56 degrees C for 30 minutes) Both Tubes A( Unheated normal serum) and B (Normal serum heated 56 degrees C for 30 minutes) Tube C (Saline) There is no control in this activity.
Observe figure and make note of the several steps in theindirect ELISA test. What four essential events are necessary todevelop a positive reaction (besides having antibody A)? Hint: Whatwould happen without rinses?
ELISAs require a number of steps to be completed in order for the test to work. Why do you need to wash your samples at each step? When you added primary antibody to the wells, what happened if your sample contained the antigen? What if it did not contain the antigen? When you added secondary antibody to the wells, what happened if your sample contained the antigen? What if it did not contain the antigen?
Why is a positive and a negative control used in a Elisa experiment?
What are the ordered steps of an ELISA protocol? A. Add primary antibody->wash-> Bind sample to a surface ->Add substrate ->Add secondary antibody-enzyme conjugate ->wash B. Bind sample to support -> Add substrate -> Add primary antibody -> wash -> Add secondary antibody-enzyme conjugate -> wash C. Bind sample to a surface -> Add primary antibody -> wash -> Add secondary antibody-enzyme conjugate -> wash -> Add substrate D. Add secondary antibody-enzyme conjugate -> wash -> Add primary antibody -> wash -> Add substrate -> Bind sample to surface
You want to subculture your cells from T25 flask to 96-well plates. You first collected your cells in a tube with 5ml of culture medium. Then carried out a trypan blue assay and counted your cells with a hematocytometer as shown in Figure below. Answer the questions according to the results: a. Calculate the concentration of the stock including the dead and living cells. Dont forget to show the units! b. Calculate the percentage (%) of the living cells in the stock. c. You want to seed 6000 living cells into each well of 96 well plates, then calculate the volume you should take from the stock for each well. 輯 1 mm I IRE