Two proteins of different molecular weight (mw) are mixed and need to be separated. When eluted through a Sephadex-G50 chromatography column, what is the order of elution (first followed by second)? A. protein with mw of 40 kDa; protein with mw of 20 kDa B. both proteins will elute at the same time C. both proteins will not elute as the combined mw is larger than 50 kDa D. protein with mw of 20 kDa; protein with mw of 40 kD

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 Two proteins of different molecular weight (mw) are mixed and need to be separated. When eluted through a Sephadex-G50 chromatography column, what is the order of elution (first followed by second)?

A. protein with mw of 40 kDa; protein with mw of 20 kDa
B. both proteins will elute at the same time
C. both proteins will not elute as the combined mw is larger than 50 kDa
D. protein with mw of 20 kDa; protein with mw of 40 kD
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