A mixture of two proteins (both with molecular mass of 50 kDa) with an isoelectric points of 7.8 and 8.9, respectively, is in a buffered solution at pH 7. Which type of liquid chromatography would be used to separate these two

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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A mixture of two proteins (both with
mass of 50 kDa) with an
molecular
isoelectric points of 7.8 and 8.9,
respectively, is in a buffered solution at pH
7. Which type of liquid chromatography
would be used to separate these two
proteins?
O a.
O b.
O C.
d.
e.
anion exchange chromatography
size-exclusion column
gel filtration
Ni-NTA column
cation exchange chromatography
Transcribed Image Text:A mixture of two proteins (both with mass of 50 kDa) with an molecular isoelectric points of 7.8 and 8.9, respectively, is in a buffered solution at pH 7. Which type of liquid chromatography would be used to separate these two proteins? O a. O b. O C. d. e. anion exchange chromatography size-exclusion column gel filtration Ni-NTA column cation exchange chromatography
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