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What kind of buffer would you make with a optimal activity at ph 4.30?

What kind of buffer would you make with a optimal activity at ph 4.30?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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What kind of buffer would you make with a optimal activity at ph 4.30?
Materials Available In the Lab DI Water Acetate pKa = 4.76 o 1.5 M acetic açid solution CH,COOH o Solid sodium acetate (molar mass 82 g/mol) NACH,CO0 Tris (pKa = 8.10) o Solid tris chloride (molar mass 157.6 g/mol) (HOCH,),NH,Cl o Solid tris base (molar mass 121.1 g/mol) (HOCH,),NH, Phosphate (pK1 = 2.14, pK2 = 6.86, pK3 = 12.4) 1.0 M phosphoric acid solution H3PO, 1.5 M monosodium phosphate solution NaH,PO, 1.25 M disodium phosphate solution Na,HPO, 2.25 M trisodium phosphate solution Na, PO, Questions 1. You are interested in studying a protein with optimal activity at pH 4.30. a. What kind of buffer would you make? Justify your answer. b. What is the weak acid? What is the conjugate base?
Transcribed Image Text:Materials Available In the Lab DI Water Acetate pKa = 4.76 o 1.5 M acetic açid solution CH,COOH o Solid sodium acetate (molar mass 82 g/mol) NACH,CO0 Tris (pKa = 8.10) o Solid tris chloride (molar mass 157.6 g/mol) (HOCH,),NH,Cl o Solid tris base (molar mass 121.1 g/mol) (HOCH,),NH, Phosphate (pK1 = 2.14, pK2 = 6.86, pK3 = 12.4) 1.0 M phosphoric acid solution H3PO, 1.5 M monosodium phosphate solution NaH,PO, 1.25 M disodium phosphate solution Na,HPO, 2.25 M trisodium phosphate solution Na, PO, Questions 1. You are interested in studying a protein with optimal activity at pH 4.30. a. What kind of buffer would you make? Justify your answer. b. What is the weak acid? What is the conjugate base?
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