Use the information on the proteins given to solve the questions:  A. Draw a UV-Vis spectrum of each protein (A, B, C, and D) from 240 – 480 nm if they were all present at 20 µM.  B. How can you create a two-step purification procedure that could be used to purify protein A from the other proteins. In addition, include a chromotagram as well.

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
icon
Related questions
Question

Use the information on the proteins given to solve the questions: 

A. Draw a UV-Vis spectrum of each protein (A, B, C, and D) from 240 – 480 nm if they were all present at 20 µM. 

B. How can you create a two-step purification procedure that could be used to purify protein A from the other proteins. In addition, include a chromotagram as well. 

 

Protein
AB
с
D
Size (kDa)
10
50
10
50
pl
4
4
8
8
ε at 280 nm
7000
14000
3000
50000
Red Colored?
Yes
No
No
No
Transcribed Image Text:Protein AB с D Size (kDa) 10 50 10 50 pl 4 4 8 8 ε at 280 nm 7000 14000 3000 50000 Red Colored? Yes No No No
Expert Solution
steps

Step by step

Solved in 4 steps

Blurred answer
Similar questions
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON