Use the information on the proteins given to solve the questions: A. Draw a UV-Vis spectrum of each protein (A, B, C, and D) from 240 – 480 nm if they were all present at 20 µM. B. How can you create a two-step purification procedure that could be used to purify protein A from the other proteins. In addition, include a chromotagram as well.
Use the information on the proteins given to solve the questions: A. Draw a UV-Vis spectrum of each protein (A, B, C, and D) from 240 – 480 nm if they were all present at 20 µM. B. How can you create a two-step purification procedure that could be used to purify protein A from the other proteins. In addition, include a chromotagram as well.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
Use the information on the proteins given to solve the questions:
A. Draw a UV-Vis spectrum of each protein (A, B, C, and D) from 240 – 480 nm if they were all present at 20 µM.
B. How can you create a two-step purification procedure that could be used to purify protein A from the other proteins. In addition, include a chromotagram as well.
![Protein
AB
с
D
Size (kDa)
10
50
10
50
pl
4
4
8
8
ε at 280 nm
7000
14000
3000
50000
Red Colored?
Yes
No
No
No](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F9b60de5f-8fb0-4e3b-8258-2de6c1a93a8c%2Feae0dad5-5fad-4031-a382-12f167551137%2Fdf8wxd8_processed.png&w=3840&q=75)
Transcribed Image Text:Protein
AB
с
D
Size (kDa)
10
50
10
50
pl
4
4
8
8
ε at 280 nm
7000
14000
3000
50000
Red Colored?
Yes
No
No
No
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