As you have seen, the protein assay relies upon the ability of proteins to bind the dye. Some substances such as dish soap can inhibit the interaction of the dye with the protein. If your unknown samples were accidentally analyzed using cuvettes that had not been well rinsed after washing, how would your calculated protein concentration be affected (Higher, Lower, No Change)? Briefly explain your reasoning.
As you have seen, the protein assay relies upon the ability of proteins to bind the dye. Some substances such as dish soap can inhibit the interaction of the dye with the protein. If your unknown samples were accidentally analyzed using cuvettes that had not been well rinsed after washing, how would your calculated protein concentration be affected (Higher, Lower, No Change)? Briefly explain your reasoning.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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As you have seen, the protein assay relies upon the ability of proteins to bind the dye. Some substances such as dish soap can inhibit the interaction of the dye with the protein. If your unknown samples were accidentally analyzed using cuvettes that had not been well rinsed after washing, how would your calculated protein concentration be affected (Higher, Lower, No Change)? Briefly explain your reasoning.
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