Analysis of a protein is taking place. The enzymic acivity of this protein is stable up to temperatures of 40 degrees celsius. ph values are between 2.5 and 11.5. Now, size exclusion chromatography is being done. Use the graph below and analytical methods to determine molecular weight of protein.
Analysis of a protein is taking place. The enzymic acivity of this protein is stable up to temperatures of 40 degrees celsius. ph values are between 2.5 and 11.5. Now, size exclusion chromatography is being done. Use the graph below and analytical methods to determine molecular weight of protein.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
Analysis of a protein is taking place. The enzymic acivity of this protein is stable up to temperatures of 40 degrees celsius. ph values are between 2.5 and 11.5. Now, size exclusion chromatography is being done. Use the graph below and analytical methods to determine molecular weight of protein.
Transcribed Image Text:Absorbance at 280 nm
2.0
1.0
0
I
Gel filtration on Sephadex G-50
M
20
30
10
40
50
60
70
Fraction number
80
90
100
110
120
Transcribed Image Text:Method
Initial
Purification of protein 3 from Easy3 Mixture
Protein (mg)
?
m
30.0
Records of subsequent purification steps will be added here.
Enzyme (Units)
2000.0
Gel filtration media
Cancel
Sephadex G-50
Sephadex G-100
Sephacryl S-200 HR
Ultrogel AcA 54
Ultrogel ACA 44
Ultrogel ACA 34
Bio-Gel P-60
Bio-Gel P-150
Bio-Gel P-300
OK
Yield (%)
100
Enrichment
1.0
Cost
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