can you tell what the pKas are from this unknown amino acid based on buffering regions and narrow down which amino acid it would be?

Q: List all the amino acids you would expect to bind to a cation-exchange column at pH 2.

A: Chromatography is derived from two Greek words, chroma means colour and graphein means to write.…

Q: Which peptide has greater absorbance at 280 nm? Why?…

A: Beer-Lambert Law provides the mathematical relation between the fraction of the incident light…

Q: Given a stock protein solution with a concentration of 3 mg/ml, determine the protein concentration…

A: Stock solutions are used in many laboratory procedures, in order to save space for keeping larger…

Q: using the pKA table, determine the Isoelectric point of Arginine

A: The isoelectric point (pI) is the pH at which the net charge on amino acid is zero. Arginine is an…

Q: What determines when an amino acid ionized? i.e. Why are both Lysine (pKa 10.53) and Argenine (pKa…

A: Amino acid are the building blocks of the protein. Each amino acid has a common basic structure with…

Q: From this standard curve and chart below, does the separation of molecules in the mixture appear…

A: Size exclusion chromatography is a technique used to separate molecules by the virtue of their…

Q: What is the purpose of a) SDS and b) beta-mercaptoethanol in the lysis buffer for proteins being…

A: Separation of proteins according to their molecular weight or size is performed by the use of…

Q: Give two benefits of utilizing the biuret reaction to assess protein concentration over directly…

A: A Biuret test is a generic test performed by molecules having two or more peptide links (CO-NH…

Q: What is the optimum pH to separate a mixture of lysine, arginine, and cysteine using…

A: Amino acid is a organic molecule made up of amino group (NH2 - basic), carboxyl group (COOH -…

Q: a) Draw a non-covalent interaction between a water molecule and the charge on a phosphorylated…

A: Unlike covalent interactions where there is sharing of electrons between two atoms, in non-covalent…

Q: HOW MANY of the following five items represent modes of protein denaturation? 1.) heating a protein…

A: Proteins are biomolecules composed of amino acids. Amino acids are joined together through peptide…

Q: (A) What property of a protein might make it difficult to transfer it from polyacrylamide gel to…

A: Nitrocellulose membranes are a popular matrix used in protein blotting because of their high…

Q: A gel filtration column with a fractionation range of 1.5-20 kDa is used to separate out the…

A: Gel filtration chromatography is a technique that is used to separate molecules based on their…

Q: Imagine that you were asked to denature a protein; you know you can do so using urea.  Your protein…

A: Denaturation is the process by which a protein looses it native structure, to the level that protein…

Q: Draw the structure of VEDMN at pH 12. What is the isoelectric point of the peptide (show how you…

A: Given peptide is composed of five amino acids so it will be called pentapeptide. The amino acids…

Q: Angiotensin Converting Enzyme (ACE) is the target of ACE inhibitors such as captopril that are used…

A: Peptides are composed of twenty standard amino acids. These twenty standard amino acids differ from…

Q: hosphate buffers are commonly used to mimic biological systems. Given that phosophoric acid is a…

A: Phosphate Buffer System consists or made up of:- Sodium  Dihydrogen Phosphate & Disodium…

Q: 1.0.1 mL of a protein solution of concentration of 11 mg/mL was diluted to a total volume of 4.0 mL…

A: The four types of biological macromolecules are nucleic acids proteins lipids and carbohydrates.…

Q: In biochemistry, what is definition of buffer , which amino acid can act as buffer? what is buffer…

A: The functioning of many molecules such as proteins, nucleic acids etc. depends on pH. The pH in…

Q: Enter an estimate for the charge on the peptide KPIVRFDEESC at pH 8.0.

A: Before calculating the isoelectric point (pI) of the peptide, we need to be thorough with the…