Na Purified protein Digest protein into small fragments using a protease. Determine the mass of these fragments with a first spectrometer. | 1652 Da 4000 Mass/charge Analyze this fragment with a second spectrometer. The peptide is fragmented from one end. Abund ance 1201 1428 1652 1008 1114 1315 1565 900 1800 Mass/charge -Asn-Ser-Asn-Leu-His-Ser- Short peptide sequences, such as this one, are used to search a database and identify the protein. FIGURE 24.6 The use of tandem mass spectrometry to deter- mine the amino acid sequence of a peptide and identify a protein. Abundance
Na Purified protein Digest protein into small fragments using a protease. Determine the mass of these fragments with a first spectrometer. | 1652 Da 4000 Mass/charge Analyze this fragment with a second spectrometer. The peptide is fragmented from one end. Abund ance 1201 1428 1652 1008 1114 1315 1565 900 1800 Mass/charge -Asn-Ser-Asn-Leu-His-Ser- Short peptide sequences, such as this one, are used to search a database and identify the protein. FIGURE 24.6 The use of tandem mass spectrometry to deter- mine the amino acid sequence of a peptide and identify a protein. Abundance
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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What is the purpose of tandem mass spectrometry?
Transcribed Image Text:Na
Purified protein
Digest protein into
small fragments
using a protease.
Determine the mass
of these fragments with
a first spectrometer.
| 1652 Da
4000
Mass/charge
Analyze this fragment with
a second spectrometer.
The peptide is fragmented
from one end.
Abund ance
Transcribed Image Text:1201
1428
1652
1008
1114
1315
1565
900
1800
Mass/charge
-Asn-Ser-Asn-Leu-His-Ser-
Short peptide sequences, such
as this one, are used to search a
database and identify the protein.
FIGURE 24.6 The use of tandem mass spectrometry to deter-
mine the amino acid sequence of a peptide and identify a protein.
Abundance
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