Na Purified protein Digest protein into small fragments using a protease. Determine the mass of these fragments with a first spectrometer. | 1652 Da 4000 Mass/charge Analyze this fragment with a second spectrometer. The peptide is fragmented from one end. Abund ance 1201 1428 1652 1008 1114 1315 1565 900 1800 Mass/charge -Asn-Ser-Asn-Leu-His-Ser- Short peptide sequences, such as this one, are used to search a database and identify the protein. FIGURE 24.6 The use of tandem mass spectrometry to deter- mine the amino acid sequence of a peptide and identify a protein. Abundance

Q: How do the individual protein techniques combine to study proteomics?

A: Proteins are organic macromolecules that make up around 50% of cellular dry weight. Proteins are…

Q: What is the importance of the 6.25 multiplication factor for protein analysis (Kjeldahl methods)?

A: The Kjeldahl method for protein measurement was designed to be used for the measurement of nitrogen…

Q: How do you verify amino acid sequences with proteins and species with BLAST?

A: Bioinformatics is an interdisciplinary field of study which has diverse application in the…

Q: How is DNA used to make a profile and how is the profile used?

A: The abbreviation DNA stands for deoxyribonucleic acid. DNA is found to be the hereditary material in…

Q: What are gene microarrays? How are microarrays used?

A: The process of forming a single double-stranded nucleic acid molecule by combining two…

Q: What is pharmacogenomics, and the role it plays on fast and slow etabolism and how it affects the…

A: Pharmacogenomics is an approach to the advanced medical science which states or aims to define a…

Q: What is Proteomics ?

A: Proteins are naturally occurring substances that are highly complex and made out of amino acids that…

Q: When using a probe to detect an organism, why is it necessary to have some idea as to the organism’s…

A: DNA is the genetic material in all living organisms. Each organism has a unique DNA sequence. Using…

Q: In the procedure called RNA sequencing (RNA-Seq), what type ofmolecule is actually sequenced?

A: Ribonucleic acid sequencing (RNA-seq) was developed by Michael Snyder and colleagues in 2008. It is…

Q: How do microarrays work?

A: A microarray is a laboratory tool used to detect the expression of thousands of genes at the same…

Q: What is oligonucleotide microarray?

A: An array is an orderly arrangement of samples where matching of known and unknown DNA samples is…

Q: What is the hybridization and synthesis process and why it is needed?

A: In the cell, there are several processes, which are required for survival and biochemical functions.…

Q: in the isolation of proteins in the experiment?

A: Protein plays an important role in every diet. Depending on their age and sex amount of protein an…

Q: I understand that microarrays are being used to define the molecular abnormality and the prognosis…

A: Leukemia and types

Q: How are molecular probes prepared?

A: Molecular probes are small segments of deoxyribonucleic acid (DNA) or ribonucleic acid (RNA), which…

Q: What is the origin of Masseter?

A: Muscle is a soft tissue in the body which primarily functions as source of power. Muscles cells…

Q: How can molecular structural analysis be accomplished with electrospray ionization mass…

A: Molecular structural analysis involves determining the arrangement and properties of atoms within a…

Q: Why are there different spec readings (OD600) for different strains of E. coli?

A: OD value means optical density value at 600nm wavelength of light. In spectroscopy the sample means…

Q: What are the limitations of doing a molecular identification of bacteria?

A: Bacteria are the small single-celled organisms. They are found almost everywhere on Earth and are…

Q: How is mass spectrometry used to identify proteins in a cell?

A: Mass spectrometry is the important method, which is used in mass determination of proteins and their…

Q: What are the DNA extraction methods? Explain each one.

A: Ans: The Deoxyribonucleic acid (DNA) needs to be extracted out of cell for further studies on which…

Q: What is mass fragmentography? How is this performed?

A: Mass fragmentography is also known by the name mass fragmentometric analysis or mass fragmentography…

Q: Explain the use of mass spectrometry (MS) ?

A: Mass spectroscopy is a laboratory analytical tool. It is used to detect the mass-to-charge ratio of…

Q: What are the principles of Chelex extraction and solid-phase DNA extraction methods?

A: To explain the principles of Chelex extraction and solid-phase DNA extraction methods The principle…

Q: What is an advantage of using an ELISA instead of a protein microarray to study a proteome? What is…

A: ELISA is an immunological assay commonly used to measure antibodies, antigens, proteins and…

Q: What is DNA fingerprinting? Define and differentiate VNTR and Probe and what is the applications of…

A: Step 1 DNA or deoxyribonucleic acid occurs in living systems and can function as genetic materials.…

Q: What is in situ hybridization used for and how  does In Situ Hybridization detect?

A: Hybridization, in general, is a process during which a moiety is formed having combined traits of…

Q: How proteins are analyzed using gel electrophoretic techniques?

A: Electrophoresis is a method of separating solutes based on their charge by applying an electric…

Q: How does the shotgun approach differ from the whole genome sequencing?

A: Biotechnology is concerned with exploitation of biological agents or their components for generating…

Q: nucleoprotein and where can you find it? How do we prepare sample of nucleoproteins for analysis?

A: Nucleoproteins are complex proteins associated with nucleic acids.

Q: What what is a nucleoprotein, and where do you look for one? How do we prepare nucleoprotein samples…

A: Nucleoproteins are nucleic acid-associated complex proteins.