Na Purified protein Digest protein into small fragments using a protease. Determine the mass of these fragments with a first spectrometer. | 1652 Da 4000 Mass/charge Analyze this fragment with a second spectrometer. The peptide is fragmented from one end. Abund ance 1201 1428 1652 1008 1114 1315 1565 900 1800 Mass/charge -Asn-Ser-Asn-Leu-His-Ser- Short peptide sequences, such as this one, are used to search a database and identify the protein. FIGURE 24.6 The use of tandem mass spectrometry to deter- mine the amino acid sequence of a peptide and identify a protein. Abundance

Q: Why do we need to use salt solution when we cloning dna?

A: Once the genomic deoxyribonucleic acid is certain to the silicon oxide membrane, the supermolecule…

Q: How do the individual protein techniques combine to study proteomics?

A: Proteins are organic macromolecules that make up around 50% of cellular dry weight. Proteins are…

Q: Once DNA is extracted, what can it be used for?

A: Genetic material is the molecules which should have certain characteristics like it should replicate…

Q: What is real-time PCR?

A: Besides tools, there a several techniques which are used in recombinant DNA technology. These…

Q: How do you verify amino acid sequences with proteins and species with BLAST?

A: Bioinformatics is an interdisciplinary field of study which has diverse application in the…

Q: write about tandem mass spectrometry.

A: Tandem mass spectrometry is a process that can be used to screen blood samples from newborns for the…

Q: Describe how to determine the quality of DNA sequences using a chromatogram .

A: A chromatogram is a visual representation of a DNA sample produced by a sequencing machine like the…

Q: How is DNA used to make a profile and how is the profile used?

A: The abbreviation DNA stands for deoxyribonucleic acid. DNA is found to be the hereditary material in…

Q: List all the components and explain their purpose in the reaction mixture used for the dideoxy DNA…

A: The method of evaluating the sequence of nucleotides (As, Ts, Cs, and Gs) in a portion of DNA is DNA…

Q: What is the role of NaCl in the DNA isolation procedure?

A: NaCl (Sodium Chloride) is used during various DNA isolation procedures. Usually, in DNA isolation,…

Q: How does Sanger sequencing differ from PCR?

A: Sanger sequencing and PCR are two unique molecular biology techniques, however they perform…

Q: After DNA fragments have been separated by gel electrophoresis, how can they be visualized?

A: Gel electrophoresis is a technique used for separating Deoxy ribonucleic acid (DNA) fragments based…

Q: Describe the two general types of protein microarrays. What aretheir possible applications?

A: A protein microarray is also known as the protein chip, is the high throughput method that is used…

Q: When using a probe to detect an organism, why is it necessary to have some idea as to the organism’s…

A: DNA is the genetic material in all living organisms. Each organism has a unique DNA sequence. Using…

Q: What is the purpose of shotgun sequencing?

A: Deoxyribonucleic acid (DNA) sequencing is the process of determining the nucleic acid sequence based…

Q: How do microarrays work?

A: A microarray is a laboratory tool used to detect the expression of thousands of genes at the same…

Q: What is oligonucleotide microarray?

A: An array is an orderly arrangement of samples where matching of known and unknown DNA samples is…

Q: What do you mean by fluorescent-labeled probes?

A: Fluorescent marking is the interaction of covalently binding fluorescent colors to biomolecules,…

Q: What is the hybridization and synthesis process and why it is needed?

A: In the cell, there are several processes, which are required for survival and biochemical functions.…

Q: Give the meanings of the following terms: genomics, functionalgenomics, and proteomics.

A: Genetics is defined as the study of genes. Genes are the functional and structural unit of life.…

Q: in the isolation of proteins in the experiment?

A: Protein plays an important role in every diet. Depending on their age and sex amount of protein an…

Q: How are molecular probes prepared?

A: Molecular probes are small segments of deoxyribonucleic acid (DNA) or ribonucleic acid (RNA), which…

Q: What is the origin of Masseter?

A: Muscle is a soft tissue in the body which primarily functions as source of power. Muscles cells…

Q: Why are there different spec readings (OD600) for different strains of E. coli?

A: OD value means optical density value at 600nm wavelength of light. In spectroscopy the sample means…

Q: What are the limitations of doing a molecular identification of bacteria?

A: Bacteria are the small single-celled organisms. They are found almost everywhere on Earth and are…

Q: What is the role of EDTA in the DNA isolation procedure?

A: DNA isolation is a laboratory technique used to obtain purified DNA from various samples, including…

Q: Where to start with microbial complete genome analysis?

A: Genomic analysis can be described as the process of recognizing, measuring, and comparing the…

Q: Describe the different types of chromatography used to purify proteins.

A: Introduction: Protein purification is a collection of procedures for isolating one or a few proteins…

Q: What are the DNA extraction methods? Explain each one.

A: Ans: The Deoxyribonucleic acid (DNA) needs to be extracted out of cell for further studies on which…

Q: What

A: INTRODUCTION:- Shotgun sequencing involves randomly breaking up DNA sequences  into lots of small…

Q: What molecule is next generation sequencing analyzing? DNA, RNA, or protein

A: What is next generation sequencing? It is the process of massive parallel sequencing of DNA…

Q: What is used to identify the size of a DNA molecule during gel electrophoresis?

A:

Q: How proteins are analyzed using gel electrophoretic techniques?

A: Electrophoresis is a method of separating solutes based on their charge by applying an electric…