Do you predict that this salt bridge will become stronger, become weaker, or be unaffected as pH increases from pH = 7.0 to pH = 7.9? The salt bridge will become stronger. The salt bridge will become weaker. The salt bridge will be unaffected. Submit Previous Answers ✓ Correct At pH 7.0 the glutamic acid (Glu) side chain will carry a charge of~-1 (at 3 pH units above the pK, for Glu, the side chain will be almost fully ionized); whereas the histidine (His) side chain will carry a charge of < +0.5 (at pH=pK₁ the charge on His would be +0.5; since pH = 7.0 is above its pKa, it will carry less (+) charge as it becomes more deprotonated). As the pH increase to 7.9, the charge on Glu will remain ~ -1 and the charge on His will decrease; thus, this salt bridge is predicted to become weaker as pH is raised from 7.0 to 7.9. ▾ Part B Justify your answer with calculations of partial charges on these amino acid side chains at pH = 7.0. (Hint: Consider lessons from Coulomb's law, and the Henderson-Hasselbalch equation.) Express your answers using two significant figures separated by a comma. [Π ΑΣΦ 3 0.00020,0.96

Do you predict that this salt bridge will become stronger, become weaker, or be unaffected as pH increases from pH = 7.0 to pH = 7.9? The salt bridge will become stronger. The salt bridge will become weaker. The salt bridge will be unaffected. Submit Previous Answers ✓ Correct At pH 7.0 the glutamic acid (Glu) side chain will carry a charge of~-1 (at 3 pH units above the pK, for Glu, the side chain will be almost fully ionized); whereas the histidine (His) side chain will carry a charge of < +0.5 (at pH=pK₁ the charge on His would be +0.5; since pH = 7.0 is above its pKa, it will carry less (+) charge as it becomes more deprotonated). As the pH increase to 7.9, the charge on Glu will remain ~ -1 and the charge on His will decrease; thus, this salt bridge is predicted to become weaker as pH is raised from 7.0 to 7.9. ▾ Part B Justify your answer with calculations of partial charges on these amino acid side chains at pH = 7.0. (Hint: Consider lessons from Coulomb's law, and the Henderson-Hasselbalch equation.) Express your answers using two significant figures separated by a comma. [Π ΑΣΦ 3 0.00020,0.96

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

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