A protein contains three 60-kD polypeptides and six 20-kD polypeptides. Each 60-kD chain is disulfide bonded to two 20-kD chains. The 100-kD units associate noncovalently to form a protein with a molecular mass of 300 kD. Which of the following is correct? a) PAGE shows one single band b) Molecular masses determined by SDS-PAGE with 2-mercaptoethanol are 20kD and 60 kD c) Molecular mass determined by gel filtration is 300 kD d) All answers are correct e) Molecular mass determined by SDS-PAGE without 2-mercaptoethanol is 100 kD can you explain the steps and calculations to solve this question?
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
A protein contains three 60-kD polypeptides and six 20-kD polypeptides. Each 60-kD chain is disulfide bonded to two 20-kD chains. The 100-kD units associate noncovalently to form a protein with a molecular mass of 300 kD. Which of the following is correct? a) PAGE shows one single band b) Molecular masses determined by SDS-PAGE with 2-mercaptoethanol are 20kD and 60 kD c) Molecular mass determined by gel filtration is 300 kD d) All answers are correct e) Molecular mass determined by SDS-PAGE without 2-mercaptoethanol is 100 kD
can you explain the steps and calculations to solve this question?
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