A chromatography column is used to separate four ami serine, alanine, and histidine. A cylindrical column is packed (65% by volume) particles. An aqueous solution containing 25 mol% of each amino acid is fed superficial solution velocity of 0.32 mm/sec. The column is operated continuou (pulse) time followed by elusion for a set period of time. Given the equilibrium amino acid, answer the following sets of questions: Amino Acid Kai Mw (g/mol Glutamic acid, R=(CH2)2COOH 1.52 147.13

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