A culture medium contains 1mM NaCl, 1mM of each essential amino acid, and 5g/L honey. Is the medium defined or undefined? How do you know?
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A culture medium contains 1mM NaCl, 1mM of each essential amino acid, and 5g/L honey. Is the medium defined or undefined? How do you know?
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- What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter culture media for a concentration of 6.5 x 106 cells/mL?What is the purpose of the vial that does not contain chara? (the solution with chara turned green showing that it is neutral) (the btb solution was yellow so it was acidic)You have 0.05 ml of kombucha that contains microbes at a concentration of 8.2 x 10^6 CFU/ml. You then add 4.95 ml fresh tea. What is the dilution? What is the final concentration of cells? Show your calculations.
- How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.based on the attached photo, compute the cfu/ml of 42 colonies at plate 10^5. show the complete solution and provide explanation.A culture medium is comprised of the following components: 0.5% peptone, 0.3% meat extract, 1.5% agar, and distilled water. If you are asked to prepare 765 mL of culture media, how much meat extract would you need?
- one lm of milk was added to 9 ml of sterile saline. 0.1ml of the diluted sample was put into a petri dish with melted agar. How many cfu/ml are in the milk if 200 colonies were counted?This is the growth curve for Clostirdium (incubated under optimal growth conditions). Use it to answer the following questions. 3 Cell concentration (million cells/ml) 2.5 2 0.2 1.5 1 0.5 0 2 The growth rate is lag log 0.25 4 0.32 0.5 units 6 million of cells per ml (Image description.: Horizontal axis is time in hours, Vertical is cell concentration in millions of cells per ml. Curve starts at 0,0, increases by 4 hours to 0.5 million cells/ml, goes up steeply over two hours from 0.5 million/ml at 4 hours to 2.5 million cells/ml at 8 hours, 8-12 hour shows an increase of 0.3 to 2,8 million cellls/ml and last 2 hours is flat at 2.8million cells/ml.) From 12 hr to 14 hr is called phase. lunar 2 8 Time (hours) 10 5 stationary 12 cells 14 death 16 million of cells per ml per hour hours million of cellsAssume that you are adding 300 microliters of 1% substrate solution per well in a 24-well plate. If we can order 5 milligrams of fibronectin for $871.00, how much would it cost to have enough to use every well of the 24-well culture plate?
- A urine sample is diluted 1:1,000. If 0.1 ml were put into a Petri dish with melted agar, how many CFU/ml are in the urine if 158 colonies grow?If you have a culture with 1 x 106 cells per ml, how could you use serial dilutions to obtain a suspension with 5 x 102 cells per ml? Show your answer using a serial dilution scheme or diagramThe nutrient broth is a basic media used for growing a broad variety of microorganisms in the laboratory. The nutrient broth consists mainly of 1.5g * L ^ - 1 extract, 3g * L ^ - 1 yeast extract and 5g * L ^ - 1 sodium chloride dissolved in distilled water. The nutrient agar is prepared by adding the agar at the desired amount into nutrient broth. Answer the following questions. marks) a) How would you prepare a 500 ml nutrient broth? b) How would you prepare 2% (w / v) nutrient agar for 1L? c) How would you prepare a 100 ml nutrient broth supplemented with 100mu * g / m * l ampicillin antibiotic? The stock concentration for ampicillin solution is 100mg / m * l .