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- If the pH indicator were left out of MacConkey agar, the medium would bea) complex.b) differential.c) defined.d) defined and differential.e) complex and differential.A disk-diffusion test using E. coli gave the following results: Disinfectant Zone of inhibition (diameter in mm) A 15 B 2.0 C 10 D 5 In the table above, which compound was the most effective against E. coli? A) A B) B C) C D) D E) The answer cannot be determined based on the information provided.Which statement among A-D is false regarding physical and chemical agents of control? A) O Effective killing of bacteria via irradiation with UV light would likely take longer than if irradiated with gamma rays or x-rays. B) O Detergent-type disinfectants exert their effect by solubilizing membranes. C) O Antiseptics are generally not as strongly concentrated as disinfectants D) O Sterilization and pasteurization are equivalent processes.
- Answer the following questions What is the purpose of the 95% ethanol step in the Gram staining procedure? (1 Mark) xiii) What is the function of the following reagents used in Gram stain: a) Crystal Violet b) Gram’s iodine c) Acetone/alcohol d) Safranin IThe difference in the colony appearance of bacteria cells growing on mannitol salt agar is detected by a) methyl blue b)methyl red c) phenol d) eosin e) salt .3.1) What are the reasons for finding Gram-positive organisms on a MacConkey agar plate? 3.2) What does it mean if the Gram-negatives on the MacConkey agar form red or colourless colonies? 4) A solution contains the following: 5% NaCl, 12 g/l of agar-agar, 0.1 M NaOH (M, = 40 g/mol). Calculate how much of each is needed for 550 ml. Show all 653 PM 18°C NO Back F4 7 8 6 4 5 P R G LO
- All except which of the following are important aspects to consider in determining the G+C content of a microorganism? a) determination of gram stain b)double stranded dna absorbs less light than single DNA strang c)he greater the G+C content, the higher the temperature required to denature DNA d) 3 hydrogren bonds between G+C content vs 2 hydrogen bonds between A and TA solution of ascorbic acid for injection is prepared for: A) Water for injection B) Purified water C) Water for injection, purified from CO2. D) Water for injection saturated with CO2.Give handwritten answer of the all three subparts of the question in image
- ). Consider a culture medium on which only gram-positive organisms such as Staphylococcus aureus colonies can grow due to an elevated NaCl level. A yellow halo surrounds the growth, indicating the bacterium fermented sugar in the medium, decreasing the pH as a result and changing the color of a pH indicator chemical. This type of medium would be referred to as a(n): A) Selective medium. B) Differential medium. C) Enrichment culture. D) Selective and differential medium. E) Differential and enrichment culture.Which disinfectant is the most effective? and why? Which disinfectant(s) is (are) bactericidal? and why?When is sterilization by filtration preferred over sterilization by heat? Why? What makes heat an effective sterilizing agent? Why are the conditions required for sterilization greater for dry heat (160-180°C for 1-2hrs) than for steam (121°C for 15min)?