Before using the top agar, maltose and MgSO4 must be added. Why are maltose and MgSO4 added after autoclaving and not before? The volume of each top agar is 5mL. The stock concentration of maltose is 20% and must be diluted to ~0.2%. What volume of stock maltose will you add to each top agar?
All recipes are given per litre unless specified.
Lambda Broth (LB) Plates Top Agar
10 g tryptone 10 g Tryptone
2.5 g NaCl 2.5 g NaCl
15 g Agar 7 g Agar
Make 5 mL aliquots, then autoclave.
Lambda Broth (LB) Media Suspension Media
10 g tryptone 5.0 g NaCl
2.5 g NaCl 2 g MgSO4· 7H2O
50 ml 1 M Tris pH 7.5
In LB medium
Before using the top agar, maltose and MgSO4 must be added. Why are maltose and MgSO4 added after autoclaving and not before? The volume of each top agar is 5mL. The stock concentration of maltose is 20% and must be diluted to ~0.2%. What volume of stock maltose will you add to each top agar?
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