A one-to-one protein (P)-ligand (L) complexation (P + L <-> PL) has a dissociationequilibrium constant (Kd) value of 100 nM at 25°C, and the Kd remains the same at 37°C.1) What is AS of binding at 25°C? Assume ACp of the binding is 0 over the temperaturerange.AS = 1.34E2kJ/(mol*K) (note the unit!!) (sig. fig =3)2) What is the concentration of the PL complex formed at equilibrium when you mix 0.20uM (microM) of Protein and 1.0 uM of Ligand together at 37°C?PL at equilibrium = 8.1E-1uM (note the unit!!) (sig. fig =2)

∆Cp is the heat capacity at constant pressure. ∆S is change in entropy, degree of randomness. At…

Answered: A one-to-one protein (P)-ligand (L)…

Biochemistry

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ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

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A one-to-one protein (P)-ligand (L) complexation (P + L <-> PL) has a equilibrium constant (Kd) value of 100 nM at 25°C, and the Kd remains the same at 37°C.