Calculate the net charge of the following peptide at pH 2, pH 6 and pH 14 (use 2 and 9 as pKa values for the carboxylic acid and amino groups, respectively, use the attached table for the side chain pKa values). Ile-Leu-Ile-Lys-Glu-Cys-His-Glu-Met-Ile-Ser-Thr-Arg-Tyr TABLE 4.1 pk, Values of Common Amino AcidsAmino Acida-COOH pkaAlanineArginineAsparagineAspartic acidCysteineGlutamic acidGlutamineGlycineHistidineIsoleucineLeucineLysineMethioninePhenylalanineProlineSerineThreonineTryptophanTyrosineValine2.42.22.02.11.72.22.22.31.82.42.42.22.31.82.12.22.62.42.22.3*****************a-NH3* pKa9.79.08.89.810.89.79.19.69.29.79.69.09.29.110.69.210.49.49.19.6R group pKa12.53.98.34.36.010.5~13~1310.1*************

The ionizable groups in a peptide include its N-terminal alpha-amino group, C-terminal…

Answered: Calculate the net charge of the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Calculate the net charge of the following peptide at pH 2, pH 6 and pH 14 (use 2 and 9 as pKa values for the carboxylic acid and amino groups, respectively, use the attached table for the side chain pKa values).

Ile-Leu-Ile-Lys-Glu-Cys-His-Glu-Met-Ile-Ser-Thr-Arg-Tyr

TABLE 4.1 pk, Values of Common Amino Acids
Amino Acid
a-COOH pka
Alanine
Arginine
Asparagine
Aspartic acid
Cysteine
Glutamic acid
Glutamine
Glycine
Histidine
Isoleucine
Leucine
Lysine
Methionine
Phenylalanine
Proline
Serine
Threonine
Tryptophan
Tyrosine
Valine
2.4
2.2
2.0
2.1
1.7
2.2
2.2
2.3
1.8
2.4
2.4
2.2
2.3
1.8
2.1
2.2
2.6
2.4
2.2
2.3
*****************
a-NH3* pKa
9.7
9.0
8.8
9.8
10.8
9.7
9.1
9.6
9.2
9.7
9.6
9.0
9.2
9.1
10.6
9.2
10.4
9.4
9.1
9.6
R group pKa
12.5
3.9
8.3
4.3
6.0
10.5
~13
~13
10.1
*************

Definition Definition Class of organic compounds that contain a carboxyl group ( - COOH ) and has a general formula R - COOH or R - CO 2 H , where R refers to the alkyl, alkenyl, aryl, or other groups. They can undergo different chemical reactions, such as acid-base reactions, esterification, and oxidation. These are essential components of living organisms, playing important roles in metabolic processes, signaling, and as pharmaceuticals.

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