Three different ligands, Ligand Q, Ligand T, and Ligand W, bind to the same protein but with different affinity:The association constant (Ka) for the binding of Ligand Q to the protein is 0.033 nM-1.The fractional saturation (Y) of the protein is 0.20 when the concentration of Ligand T is 1.25 nM.The fractional saturation (Y) of the protein is 0.80 when the concentration of Ligand W is 72 nM.Given this information, Calculate Kd for the binding of each ligand to this protein.Which ligand binds with greatest affinity?Which ligand binds with the lowest affinity?

Equilibrium constant or affinity constant Ka is defined as the ratio of the concentration of protein…

Answered: Three different ligands, Ligand Q,…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Three different ligands, Ligand Q, Ligand T, and Ligand W, bind to the same protein but with different affinity:

The association constant (K_a) for the binding of Ligand Q to the protein is 0.033 nM^-1.
The fractional saturation (Y) of the protein is 0.20 when the concentration of Ligand T is 1.25 nM.
The fractional saturation (Y) of the protein is 0.80 when the concentration of Ligand W is 72 nM.

Given this information,

Calculate K_d for the binding of each ligand to this protein.
Which ligand binds with greatest affinity?
Which ligand binds with the lowest affinity?

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